US 12,030,957 B2
PLAP-CAR-effector cells
Lijun Wu, Albany, CA (US); and Vita Golubovskaya, Richmond, CA (US)
Assigned to ProMab Biotechnologies, Inc., Richmond, CA (US); and Forevertek Biotechnology Co., Ltd, Changsha (CN)
Filed by ProMab Biotechnologies, Inc., Richmond, CA (US); and Forevertek Biotechnology Co., Ltd, Hunan (CN)
Filed on Dec. 8, 2020, as Appl. No. 17/115,591.
Application 17/115,591 is a continuation of application No. PCT/US2019/033953, filed on May 24, 2019.
Claims priority of provisional application 62/792,344, filed on Jan. 14, 2019.
Claims priority of provisional application 62/683,999, filed on Jun. 12, 2018.
Prior Publication US 2021/0107996 A1, Apr. 15, 2021
Int. Cl. C07K 14/725 (2006.01); A61K 35/17 (2015.01); A61P 35/00 (2006.01); C07K 14/705 (2006.01); C07K 16/28 (2006.01); C07K 16/40 (2006.01); A61K 39/00 (2006.01)
CPC C07K 16/40 (2013.01) [A61K 35/17 (2013.01); A61P 35/00 (2018.01); C07K 14/7051 (2013.01); C07K 14/70517 (2013.01); C07K 14/70521 (2013.01); C07K 16/2803 (2013.01); C07K 16/2818 (2013.01); C07K 16/2827 (2013.01); A61K 2039/505 (2013.01); A61K 2039/5156 (2013.01); A61K 2039/5158 (2013.01); C07K 2317/24 (2013.01); C07K 2317/567 (2013.01); C07K 2317/622 (2013.01); C07K 2317/76 (2013.01); C07K 2319/02 (2013.01); C07K 2319/03 (2013.01); C07K 2319/30 (2013.01); C07K 2319/33 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A chimeric antigen receptor (CAR) comprising from N-terminus to C-terminus:
(i) a single-chain variable fragment (scFv) comprising VH and VL, wherein scFv binds to PLAP (placental alkaline phosphatase), VH has the amino acid sequence of SEQ ID NO: 21, and VL has the amino acid sequence of SEQ ID NO: 22,
(ii) a transmembrane domain,
(iii) a co-stimulatory domain of CD28, OX-40, GITR, or 4-1BB, and
(iv) an activating domain.