	US 12,030,933 B2
	Anti-bed bug monoclonal antibodies and methods of making and uses thereof
William John Hall, Harrisonburg, VA (US); and Min Wang, San Diego, CA (US)
Assigned to Redcoat Solutions, Inc., Harrisonburg, VA (US)
Filed by Redcoat Solutions, Inc., Harrisonburg, VA (US)
Filed on Jul. 29, 2020, as Appl. No. 16/942,026.
Application 16/942,026 is a continuation of application No. 15/331,632, filed on Oct. 21, 2016, granted, now 10,768,172.
Claims priority of provisional application 62/244,189, filed on Oct. 21, 2015.
Prior Publication US 2020/0363405 A1, Nov. 19, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/53 (2006.01); B22F 1/0545 (2022.01); C07K 16/00 (2006.01); C07K 16/18 (2006.01); C12N 5/12 (2006.01); C12N 5/16 (2006.01); G01N 33/543 (2006.01); G01N 33/577 (2006.01); B01J 13/00 (2006.01)

CPC C07K 16/18 (2013.01) [B22F 1/0545 (2022.01); C07K 16/00 (2013.01); C12N 5/12 (2013.01); C12N 5/163 (2013.01); G01N 33/5308 (2013.01); G01N 33/54388 (2021.08); G01N 33/577 (2013.01); B01J 13/0043 (2013.01); B22F 2301/255 (2013.01); B22F 2304/054 (2013.01); B22F 2304/056 (2013.01); C07K 2317/14 (2013.01); C07K 2317/56 (2013.01); C07K 2317/565 (2013.01); G01N 2333/43552 (2013.01)]

21 Claims

1. A conjugated monoclonal antibody or a conjugated antigen-binding fragment, comprising the three heavy and three light chain complementarity determining regions of an antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644 [BB2] or PTA-122645 [BB7], and a detection agent.

5. A conjugated monoclonal antibody or a conjugated antigen-binding fragment comprising an antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122644 [BB2], or an antigen-binding fragment thereof, and a detection agent comprising an enzymatic label, a radiolabel, a fluorophore, a chromophore, an imaging agent, a metal, a metal ion, colloidal gold, or gold nanoparticles.

6. A conjugated monoclonal antibody or a conjugated antigen-binding fragment comprising an antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122645 [BB7], or an antigen-binding fragment thereof, and a detection agent comprising an enzymatic label, a radiolabel, a fluorophore, a chromophore, an imaging agent, a metal, a metal ion, colloidal gold, or gold nanoparticles.