	US 12,030,919 B2
	Composite materials comprising synthetic dragline spider silk
Shmulik Ittah, Mevaseret Zion (IL); Meni Shimel, Kiryat Ono (IL); Ella Sklan, Jerusalem (IL); and Dganit Stern, Kibbutz Lavi (IL)
Assigned to SEEVIX MATERIAL SCIENCES LTD., Jerusalem (IL)
Filed by SEEVIX MATERIAL SCIENCES LTD., Jerusalem (IL)
Filed on May 30, 2022, as Appl. No. 17/827,920.
Application 17/827,920 is a continuation of application No. 16/076,770, granted, now 11,345,727, previously published as PCT/IL2017/050175, filed on Feb. 12, 2017.
Claims priority of provisional application 62/317,572, filed on Apr. 3, 2016.
Claims priority of provisional application 62/293,880, filed on Feb. 11, 2016.
Claims priority of application No. PCT/IL2016/050874 (WO), filed on Aug. 10, 2016.
Prior Publication US 2023/0042322 A1, Feb. 9, 2023
Int. Cl. C07K 14/435 (2006.01); C12N 5/00 (2006.01); C12N 5/071 (2010.01); D01D 5/00 (2006.01); D01F 1/10 (2006.01); D01F 4/00 (2006.01); D01F 4/02 (2006.01); D01F 6/88 (2006.01)

CPC C07K 14/43518 (2013.01) [C12N 5/0068 (2013.01); C12N 5/0602 (2013.01); D01D 5/0038 (2013.01); D01F 1/10 (2013.01); D01F 4/00 (2013.01); D01F 4/02 (2013.01); D01F 6/88 (2013.01); C12N 2533/30 (2013.01); C12N 2533/50 (2013.01); D10B 2211/01 (2013.01); D10B 2211/04 (2013.01); D10B 2331/00 (2013.01); D10B 2331/02 (2013.01); D10B 2331/041 (2013.01); D10B 2331/10 (2013.01); D10B 2401/061 (2013.01); D10B 2401/063 (2013.01)]

4 Claims

1. A composition comprising: (a) a fiber comprising “m” types of proteins of differing molecular weight, wherein each protein in said “m” proteins comprises, independently, “n” repeats of a repetitive sequence of a major ampullate spidroin (MaSp) protein, wherein m and n are, independently, an integer between 2 to 70, said protein are of differing molecular weight and a different number of said repetitive sequence, said repetitive sequence comprises an amino acid sequence as set forth in SEQ ID NO: 1: (X₁)_ZX₂GPGGYGPX₃X₄X₅GPX₆GX₇GGX₈GPGGPGX₉X₁₀, wherein X₁is, independently, at each instance A or G wherein at least 50% of (X₁)Z is A, Z is an integer between 5 to 30; X₂is S or G; X₃is G or E; X₄is G, S or N; X₅is Q or Y; X₆is G or S; X₇is P or R; X₈is Y or Q; X₉is G or S; and X₁₀is S or G; and (b) a biocompatible material, cell culture media, cells, or any combination thereof.