	US 12,030,918 B2
	Fibrin particles and methods of forming fibrin particles
John Oakey, Laramie, WY (US); and Alan Stenquist, Laramie, WY (US)
Assigned to UNIVERSITY OF WYOMING, Laramie, WY (US)
Filed by UNIVERSITY OF WYOMING, Laramie, WY (US)
Filed on Dec. 4, 2020, as Appl. No. 17/112,661.
Claims priority of provisional application 62/944,399, filed on Dec. 6, 2019.
Prior Publication US 2021/0171587 A1, Jun. 10, 2021
Int. Cl. A61K 38/00 (2006.01); C07K 14/435 (2006.01); C07K 14/75 (2006.01)

CPC C07K 14/435 (2013.01) [C07K 14/75 (2013.01)]

20 Claims

1. A method of forming fibrin particles, comprising:

(a) introducing a buffer, a fibrinogen solution, and a thrombin solution to a first end of a microfluidic device to form a mixture, the buffer comprising amino acids, the amino acids comprising:

a first amino acid having a side chain group that is positively charged at a pH of 7; and

a second amino acid having a side chain group that is negatively charged at a pH of 7;

(b) contacting the mixture with a fluorocarbon oil and a surfactant to form fibrinogen-containing particles;

(c) applying positive pressure to the microfluidic device to cause the fibrinogen-containing particles to flow towards a second end of the microfluidic device;

performing (a), (b), and (c) at a temperature that is from about 15° C. to about 25° C.;

collecting the fibrinogen-containing particles from the second end of the microfluidic device; and

polymerizing the fibrinogen-containing particles to form fibrin particles after the fibrinogen-containing particles are collected from the microfluidic device.