	US 12,030,917 B2
	Photoresponsive protein and utilization thereof
Shunta Shigemura, Nagoya (JP); Shoko Hososhima, Nagoya (JP); Satoshi Tsunoda, Nagoya (JP); and Hideki Kandori, Nagoya (JP)
Assigned to NAGOYA INSTITUTE OF TECHNOLOGY, Nagoya (JP)
Appl. No. 17/276,399
Filed by NAGOYA INSTITUTE OF TECHNOLOGY, Nagoya (JP)
PCT Filed Sep. 13, 2019, PCT No. PCT/JP2019/036230 § 371(c)(1), (2) Date Mar. 15, 2021, PCT Pub. No. WO2020/054868, PCT Pub. Date Mar. 19, 2020.
Claims priority of application No. 2018-172990 (JP), filed on Sep. 14, 2018.
Prior Publication US 2022/0073571 A1, Mar. 10, 2022
Int. Cl. C07K 14/405 (2006.01); A61K 38/00 (2006.01); A61P 27/02 (2006.01); G01N 33/68 (2006.01)

CPC C07K 14/405 (2013.01) [A61P 27/02 (2018.01); G01N 33/68 (2013.01); A61K 38/00 (2013.01); G01N 2333/405 (2013.01)]

6 Claims

1. A protein composed of the amino acid sequence represented by SEQ ID NO: 1 with one or more mutations and having channel activity, wherein:

the one or more mutations comprise one or more amino acid substitutions selected from the group consisting of L53A, L53N, E76Q, V83A, V83T, A87S, K137A, L146A, K198A, K204A, G216S, S230E, and Q231L and optionally further comprise a deletion, substitution, or insertion of one or two amino acid residues, and

the amino acid sequence of the protein has 90% or more identity with the amino acid sequence represented by SEQ ID NO: 1.