US 12,030,910 B2
Compounds and methods for the modulation of proteins
Stanley T. Crooke, Carlsbad, CA (US); Xue-hai Liang, Del Mar, CA (US); and Wen Shen, Carlsbad, CA (US)
Assigned to Ionis Pharmaceuticals, Inc., Carlsbad, CA (US)
Filed by Ionis Pharmaceuticals, Inc., Carlsbad, CA (US)
Filed on Sep. 15, 2020, as Appl. No. 17/021,510.
Application 17/021,510 is a division of application No. 15/525,232, granted, now 10,822,369, previously published as PCT/US2015/060938, filed on Nov. 16, 2015.
Claims priority of provisional application 62/233,183, filed on Sep. 25, 2015.
Claims priority of provisional application 62/156,812, filed on May 4, 2015.
Claims priority of provisional application 62/156,139, filed on May 1, 2015.
Claims priority of provisional application 62/139,626, filed on Mar. 27, 2015.
Claims priority of provisional application 62/080,223, filed on Nov. 14, 2014.
Prior Publication US 2021/0269472 A1, Sep. 2, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07H 21/04 (2006.01); A61K 31/7088 (2006.01); C07H 21/00 (2006.01); C12N 15/113 (2010.01); C12N 15/67 (2006.01); C12P 19/34 (2006.01)
CPC C07H 21/04 (2013.01) [A61K 31/7088 (2013.01); C07H 21/00 (2013.01); C12N 15/113 (2013.01); C12N 15/1137 (2013.01); C12N 15/1138 (2013.01); C12N 15/67 (2013.01); C12P 19/34 (2013.01); C12N 2310/11 (2013.01); C12N 2310/315 (2013.01); C12N 2310/3341 (2013.01); C12N 2310/346 (2013.01); C12Y 301/26004 (2013.01)] 18 Claims
 
1. A method of increasing the amount a target protein in a cell, comprising contacting the cell with an antisense compound comprising a modified oligonucleotide, wherein the target protein is encoded by a target transcript comprising at least one translation suppression element in the 5′ untranslated region of the target transcript that is a stem-loop structure, wherein the modified oligonucleotide is complementary to a target site within the stem-loop structure; and thereby increasing expression of the target protein in the cell.