US 12,030,051 B2
System and method for capturing and analyzing cells
Kalyan Handique, Ann Arbor, MI (US); Kyle Gleason, Brighton, MI (US); Austin Payne, Ypsilanti, MI (US); Priyadarshini Gogoi, Ann Arbor, MI (US); Christopher Siemer, Ypsilanti, MI (US); Yi Zhou, Plymouth, MI (US); and Saedeh Javdani Sepehri, Ypsilanti, MI (US)
Assigned to Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed by Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed on May 22, 2019, as Appl. No. 16/419,485.
Application 15/432,050 is a division of application No. 15/074,054, filed on Mar. 18, 2016, granted, now 9,707,562, issued on Jul. 18, 2017.
Application 16/419,485 is a continuation of application No. 15/782,270, filed on Oct. 12, 2017, granted, now 10,350,601.
Application 15/782,270 is a continuation of application No. 15/432,050, filed on Feb. 14, 2017, granted, now 9,821,311, issued on Nov. 21, 2017.
Application 15/074,054 is a continuation in part of application No. 14/863,191, filed on Sep. 23, 2015, granted, now 9,610,581, issued on Apr. 4, 2017.
Application 14/863,191 is a continuation of application No. 14/208,298, filed on Mar. 13, 2014, granted, now 9,174,216, issued on Nov. 3, 2015.
Claims priority of provisional application 62/136,143, filed on Mar. 20, 2015.
Claims priority of provisional application 61/894,150, filed on Oct. 22, 2013.
Claims priority of provisional application 61/829,528, filed on May 31, 2013.
Claims priority of provisional application 61/779,049, filed on Mar. 13, 2013.
Prior Publication US 2019/0270085 A1, Sep. 5, 2019
Int. Cl. B01L 3/00 (2006.01); B01L 7/00 (2006.01); G01N 35/00 (2006.01)
CPC B01L 3/502761 (2013.01) [B01L 3/502715 (2013.01); B01L 3/527 (2013.01); B01L 3/5635 (2013.01); B01L 7/00 (2013.01); G01N 35/0098 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0652 (2013.01); B01L 2200/0684 (2013.01); B01L 2300/06 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0832 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/1822 (2013.01); B01L 2300/1827 (2013.01); B01L 2300/185 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0683 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A system for fluid delivery, the system comprising:
a reservoir;
a microfluidic chip comprising a set of pores configured to capture and isolate cells in single-cell format;
a manifold supporting the microfluidic chip and comprising:
a manifold inlet and a manifold outlet, the manifold inlet fluidly coupled with the reservoir,
a fluid delivery region recessed into the manifold and positioned between the manifold inlet and the manifold outlet, the fluid delivery region spanning the surface of the microfluidic chip, and
a set of openings defined at the manifold, the set of openings comprising:
a set of outlets fluidly coupled to the manifold inlet by a first set of branching channels, and
a set of inlets fluidly coupled to the manifold outlet by a second set of branching channels,
wherein the set of outlets with the first set of branching channels and the set of inlets with the second set of branching channels are symmetrically opposed across the fluid delivery region, without direct coupling between the set of outlets and the set of inlets by microfluidic channels, and
wherein the first and the second sets of branching channels drive uniform distribution of flow from the set of outlets, across the surface of the microfluidic chip when the microfluidic chip is interfaced with the manifold, and to the set of inlets;
a pump comprising computer-driven architecture for driving fluid from the reservoir into the manifold inlet in a forward direction and from the manifold inlet into the reservoir in a reverse direction;
a magnet positioned proximal the reservoir and the manifold inlet, wherein the magnet is configured to retain a concentration of contaminating particles within the reservoir, said contaminating particles originating from flow driven from the microfluidic chip and into the reservoir in the reverse direction;
a seal between the microfluidic chip and the manifold; and
a bubble removal module configured to remove bubbles from the system and comprising an outlet reservoir coupled to the manifold outlet, wherein the pump is coupled to the outlet reservoir and comprises computer-driven architecture for reversing application of negative pressure and positive pressure to drive fluid in the forward direction and the reverse direction between the reservoir, the set of pores of the microfluid chip, and the outlet reservoir.