US 12,030,048 B2
Microfluidic chip
Linfen Yu, Guangdong (CN); and Wei Yang, Guangdong (CN)
Assigned to SHENZHEN BIORAIN TECHNOLOGY CO., LTD., Shenzhen (CN)
Appl. No. 17/292,716
Filed by SHENZHEN BIORAIN TECHNOLOGY CO., LTD., Guangdong (CN)
PCT Filed Mar. 6, 2019, PCT No. PCT/CN2019/077082
§ 371(c)(1), (2) Date May 10, 2021,
PCT Pub. No. WO2020/177088, PCT Pub. Date Sep. 10, 2020.
Claims priority of application No. 201910158935.5 (CN), filed on Mar. 1, 2019.
Prior Publication US 2022/0001381 A1, Jan. 6, 2022
Int. Cl. B01L 3/00 (2006.01); B01F 23/00 (2022.01); B01F 23/41 (2022.01); B01F 101/23 (2022.01); B23Q 17/24 (2006.01); C12M 1/34 (2006.01); C12Q 1/04 (2006.01); C12Q 1/18 (2006.01); C12Q 1/686 (2018.01); G01N 21/3577 (2014.01); G01N 21/359 (2014.01); G01N 21/39 (2006.01); G01N 21/45 (2006.01); G01N 21/64 (2006.01); G01N 30/12 (2006.01); G01N 30/68 (2006.01); G01N 30/70 (2006.01); G01N 30/72 (2006.01); G01N 30/88 (2006.01); G01N 33/00 (2006.01); G01N 33/18 (2006.01); G01N 33/50 (2006.01); G01N 33/68 (2006.01)
CPC B01L 3/50273 (2013.01) [B01L 3/502715 (2013.01); B01L 3/502753 (2013.01); B01L 3/502784 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/0689 (2013.01); B01L 2300/04 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0681 (2013.01); C12Q 1/686 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A microfluidic chip, comprising a chip upper cover, a chip lower layer, a membrane, a sealing gasket and a sealing ring; wherein a lower surface of the chip upper cover fits to an upper surface of the chip lower layer, and a lower surface of the chip lower layer fits to an upper surface of the membrane;
the microfluidic chip is provided with a sample storage zone, a droplet formation zone, a droplet storage zone, a droplet detection zone and a waste liquid storage zone, the sample storage zone and the waste liquid storage zone are arranged on the lower surface of the chip upper cover, the droplet formation zone, the droplet storage zone and the droplet detection zone are arranged on the lower surface of the chip lower layer, and communication between the sample storage zone and the droplet formation zone, communication between the droplet formation zone and the droplet storage zone, communication between the droplet storage zone and the droplet detection zone, and communication between the droplet detection zone and the waste liquid storage zone are all realized by means of a micro-channel or a micropore;
the microfluidic chip is horizontally placed during sample loading; and the microfluidic chip is vertically or obliquely placed during droplet formation, PCR reaction and droplet detection, wherein the droplet formation zone is located at the lower end of the microfluidic chip, and the waste liquid storage zone is located at the upper end of the microfluidic chip;
wherein the sealing gasket is symmetrically provided with a formation continuous phase injection orifice and a detection continuous phase injection orifice alternately, the formation continuous phase injection orifice is connected with the detection continuous phase injection orifice by means of a connecting section, and the left end and the right end of the sealing gasket are provided with formation continuous phase injection orifices; the upper ends and the lower ends of the formation continuous phase injection orifice and the detection continuous phase injection orifice are each provided with a first annular seal.