CPC B01D 15/22 (2013.01) [B01D 15/206 (2013.01); B01D 15/424 (2013.01); C12M 47/10 (2013.01); G01N 30/56 (2013.01); G01N 30/606 (2013.01); G01N 2030/562 (2013.01)] | 24 Claims |
1. A method for separating at least one target compound from a feed solution, the method comprising:
filling a bioprocess package with a chromatography resin, the bioprocess package comprising:
a 2D flexible container comprising an interior compartment, a height having an upper half and a lower half, an inlet and an outlet, the inlet and the outlet being disposed on the same half of the 2D flexible container; and
a channel-forming feature in the interior compartment of the container, the channel-forming feature being configured such that a fluid flow path provided by the channel-forming feature is in fluid communication with the outlet;
wherein the channel-forming feature is on the same half of the 2D flexible container as the outlet; and
wherein the channel-forming feature and the outlet are separate components;
flowing a feed solution into the bioprocess package to contact the chromatography resin such that substantially all of the at least one target compound binds to the chromatography resin;
washing the chromatography resin in the bioprocess package; and
eluting the chromatography resin such that substantially all of the at least one target compound is released from the chromatography resin.
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