US 12,029,999 B2
Package for batch chromatography
Vasiliy Nikolaevich Goral, Painted Post, NY (US); and Ryann Loren Russell, Drexel Hill, PA (US)
Assigned to CORNING INCORPORATED, Corning, NY (US)
Appl. No. 16/766,038
Filed by CORNING INCORPORATED, Corning, NY (US)
PCT Filed Nov. 30, 2018, PCT No. PCT/US2018/063203
§ 371(c)(1), (2) Date May 21, 2020,
PCT Pub. No. WO2019/108874, PCT Pub. Date Jun. 6, 2019.
Claims priority of provisional application 62/640,190, filed on Mar. 8, 2018.
Claims priority of provisional application 62/592,983, filed on Nov. 30, 2017.
Prior Publication US 2020/0360838 A1, Nov. 19, 2020
Int. Cl. B01D 15/22 (2006.01); B01D 15/20 (2006.01); B01D 15/42 (2006.01); C12M 1/00 (2006.01); G01N 30/56 (2006.01); G01N 30/60 (2006.01)
CPC B01D 15/22 (2013.01) [B01D 15/206 (2013.01); B01D 15/424 (2013.01); C12M 47/10 (2013.01); G01N 30/56 (2013.01); G01N 30/606 (2013.01); G01N 2030/562 (2013.01)] 24 Claims
OG exemplary drawing
 
1. A method for separating at least one target compound from a feed solution, the method comprising:
filling a bioprocess package with a chromatography resin, the bioprocess package comprising:
a 2D flexible container comprising an interior compartment, a height having an upper half and a lower half, an inlet and an outlet, the inlet and the outlet being disposed on the same half of the 2D flexible container; and
a channel-forming feature in the interior compartment of the container, the channel-forming feature being configured such that a fluid flow path provided by the channel-forming feature is in fluid communication with the outlet;
wherein the channel-forming feature is on the same half of the 2D flexible container as the outlet; and
wherein the channel-forming feature and the outlet are separate components;
flowing a feed solution into the bioprocess package to contact the chromatography resin such that substantially all of the at least one target compound binds to the chromatography resin;
washing the chromatography resin in the bioprocess package; and
eluting the chromatography resin such that substantially all of the at least one target compound is released from the chromatography resin.