	US 12,029,764 B2
	Compositions and methods for obtaining organoids
Charis-Patricia Segeritz, Vancouver (CA); Ryan Conder, Vancouver (CA); and Michael Riedel, Vancouver (CA)
Assigned to STEMCELL TECHNOLOGIES CANADA INC., Vancouver (CA)
Appl. No. 16/618,148
Filed by STEMCELL TECHNOLOGIES CANADA INC., Vancouver (CA)
PCT Filed May 29, 2018, PCT No. PCT/CA2018/050625 § 371(c)(1), (2) Date Nov. 28, 2019, PCT Pub. No. WO2018/218344, PCT Pub. Date Dec. 6, 2018.
Claims priority of provisional application 62/512,138, filed on May 29, 2017.
Prior Publication US 2020/0188443 A1, Jun. 18, 2020
Int. Cl. A61K 35/38 (2015.01); A61L 27/36 (2006.01); A61L 27/38 (2006.01); A61L 27/54 (2006.01); G01N 33/48 (2006.01)

CPC A61K 35/38 (2013.01) [A61L 27/3633 (2013.01); A61L 27/3813 (2013.01); A61L 27/54 (2013.01); A61L 2300/434 (2013.01); A61L 2430/28 (2013.01); G01N 33/48 (2013.01)]

8 Claims

1. A medium for obtaining an epithelial organoid, the medium comprising a basal medium and a Wnt agonist, wherein the medium is (a) free from FGF and/or nicotinamide and (b) free from an inhibitor of BMP signalling and wherein the medium is capable of supporting the organoids for 5 or more passages.