US 12,029,638 B2
Systems and methods for producing gastrointestinal tissues
Saverio La Francesca, Houston, TX (US); Sherif Soliman, Holliston, MA (US); Matthew Marsh, Holliston, MA (US); Shunfu Hu, Holliston, MA (US); and Linghui Meng, Holliston, MA (US)
Assigned to HARVARD APPARATUS REGENERATIVE TECHNOLOGY, INC, Holliston, MA (US)
Filed by Biostage, Inc., Holliston, MA (US)
Filed on Jan. 31, 2022, as Appl. No. 17/589,096.
Application 17/589,096 is a continuation of application No. 16/391,212, filed on Apr. 22, 2019, granted, now 11,234,805.
Application 16/391,212 is a continuation of application No. 16/020,053, filed on Jun. 27, 2018.
Application 16/020,053 is a continuation of application No. 15/350,970, filed on Nov. 14, 2016, granted, now 10,265,153, issued on Apr. 23, 2019.
Application 16/391,212 is a continuation of application No. 15/350,970, filed on Nov. 14, 2016, granted, now 10,265,153, issued on Apr. 23, 2019.
Claims priority of provisional application 62/276,715, filed on Jan. 8, 2016.
Claims priority of provisional application 62/254,700, filed on Nov. 12, 2015.
Prior Publication US 2022/0226097 A1, Jul. 21, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A61F 2/04 (2013.01); A61F 2/00 (2006.01); A61F 2/07 (2013.01); A61F 2/82 (2013.01); A61L 27/04 (2006.01); A61L 27/18 (2006.01); A61L 27/38 (2006.01); A61L 27/56 (2006.01); A61L 27/58 (2006.01); C12M 1/12 (2006.01); C12M 3/00 (2006.01); A61F 2/90 (2013.01)
CPC A61F 2/04 (2013.01) [A61F 2/0077 (2013.01); A61F 2/07 (2013.01); A61F 2/82 (2013.01); A61L 27/04 (2013.01); A61L 27/18 (2013.01); A61L 27/3834 (2013.01); A61L 27/56 (2013.01); A61L 27/58 (2013.01); C12M 21/08 (2013.01); C12M 25/14 (2013.01); A61F 2002/0086 (2013.01); A61F 2002/043 (2013.01); A61F 2002/044 (2013.01); A61F 2002/045 (2013.01); A61F 2002/046 (2013.01); A61F 2/90 (2013.01); A61F 2210/0004 (2013.01); A61F 2210/0076 (2013.01); A61F 2230/0069 (2013.01); A61F 2250/0067 (2013.01); A61L 2430/22 (2013.01)] 24 Claims
OG exemplary drawing
 
1. A method, comprising the steps of:
resecting a portion of a tubular organ in a subject, the resecting step producing a resected organ portion, the resected organ portion having at least one resection edge having tissue and remaining in the subject at a resection site;
implanting a synthetic polymeric scaffold at the resection site to form an implantation site, the synthetic polymeric scaffold including a body section having an outer polymeric surface, wherein the outer polymeric surface includes spun polymeric fibers, and a cellularized sheath layer overlying at least a portion of the outer polymeric surface of the body section;
maintaining the synthetic polymeric scaffold at the implantation site formed at the resection site for a period of time sufficient to achieve guided tissue growth along the synthetic polymeric scaffold, the guided tissue growth derived from and in contact with the tissue at the at least one resection edge present in the resected organ portion remaining in the subject at the resection site; and
after achieving the guided tissue growth, intrascopically removing the synthetic polymeric scaffold from the implantation site, the removing step occurring in a manner such that the guided tissue growth remains in contact with the resected organ portion of the tubular organ remaining in the subject,
wherein the guided tissue growth achieved comprises multilayered muscle tissue and epithelia configured as a lumen.