US 11,698,366 B2
Human tissue stem cell and use thereof
Toshiro Sato, Tokyo (JP); and Mariko Shimokawa, Tokyo (JP)
Assigned to KEIO UNIVERSITY, Tokyo (JP)
Appl. No. 16/497,659
Filed by KEIO UNIVERSITY, Tokyo (JP)
PCT Filed Mar. 27, 2018, PCT No. PCT/JP2018/012356
§ 371(c)(1), (2) Date Sep. 25, 2019,
PCT Pub. No. WO2018/181276, PCT Pub. Date Oct. 4, 2018.
Claims priority of application No. 2017-063171 (JP), filed on Mar. 28, 2017.
Prior Publication US 2021/0102934 A1, Apr. 8, 2021
Int. Cl. G01N 33/50 (2006.01); C07K 14/705 (2006.01); C12N 5/095 (2010.01); C12N 5/10 (2006.01); C12N 15/65 (2006.01); C12Q 1/6897 (2018.01); G01N 33/574 (2006.01)
CPC G01N 33/5011 (2013.01) [C07K 14/705 (2013.01); C12N 5/0695 (2013.01); C12N 5/10 (2013.01); C12N 15/65 (2013.01); C12Q 1/6897 (2013.01); G01N 33/574 (2013.01)] 4 Claims
 
1. A human tissue stem cell in which a gene of interest is introduced into a gene locus of a stem cell marker gene,
wherein the stem cell marker gene is a Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 5 (LGR5) gene and
wherein the gene of interest is introduced into exon 18 of an LGR5 gene locus.