US 11,698,364 B2
Real-time cell-surface marker detection
Thomas J. Matula, Kirkland, WA (US); Masaoki Kawasumi, Seattle, WA (US); and Oleg Sapozhnikov, Seattle, WA (US)
Assigned to University of Washington, Seattle, WA (US)
Filed by University of Washington, Seattle, WA (US)
Filed on Jun. 27, 2019, as Appl. No. 16/455,550.
Claims priority of provisional application 62/690,452, filed on Jun. 27, 2018.
Prior Publication US 2020/0001293 A1, Jan. 2, 2020
Int. Cl. G01N 33/49 (2006.01); B01L 3/00 (2006.01); G01N 33/58 (2006.01); G01N 15/10 (2006.01); G01N 33/50 (2006.01); G01N 15/14 (2006.01)
CPC G01N 33/4915 (2013.01) [B01L 3/50273 (2013.01); B01L 3/502761 (2013.01); G01N 15/1056 (2013.01); G01N 33/5014 (2013.01); G01N 33/585 (2013.01); B01L 2200/0647 (2013.01); B01L 2400/0436 (2013.01); G01N 2015/142 (2013.01); G01N 2510/00 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A cell-sorting system comprising:
a flow cell for flowing a sample comprising microbubble-labeled cells and unlabeled cells, wherein the flow cell comprises one or more inlet channels, a flow channel having an upstream portion, upstream of one or more acoustic transducers relative to a direction of sample flow through the flow channel, and a downstream portion, downstream of the one or more acoustic transducers relative to a direction of sample flow through the flow channel, and one or more outlet channels; and
the one or more acoustic transducers acoustically coupled with the flow cell, wherein the one or more acoustic transducers is positioned and configured to deliver a traveling acoustic wave through the flow channel to apply an acoustic radiation force to a sample flowing through the flow channel such that the microbubble-labeled cells are displaced relative to unlabeled cells along a direction of the acoustic radiation force away from the one or more acoustic transducers, the direction of the acoustic radiation force being transverse to the direction of sample flow.