US 11,697,819 B2
Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells
Oded Shoseyov, Karme Yosef (IL); Helena Magrisso, Rehovot (IL); Tzvi Zvirin, Holon (IL); Amit Yaari, Yizrael (IL); and Zohar Katz, Modiin-Maccabim-Reut (IL)
Assigned to Yissum Research Development Company of the Hebrew University of Jerusalem Ltd, Jerusalem (IL)
Filed by Yissum Research Development Company of the Hebrew University of Jerusalem Ltd., Jerusalem (IL)
Filed on Jun. 11, 2019, as Appl. No. 16/437,844.
Application 16/437,844 is a continuation of application No. 15/600,193, filed on May 19, 2017.
Application 15/600,193 is a continuation of application No. PCT/IL2015/051112, filed on Nov. 19, 2015.
Claims priority of provisional application 62/082,204, filed on Nov. 20, 2014.
Prior Publication US 2019/0382781 A1, Dec. 19, 2019
Int. Cl. C12N 15/82 (2006.01); C12N 15/62 (2006.01); C12P 21/00 (2006.01); C07K 16/22 (2006.01); C07K 16/24 (2006.01); C12N 9/24 (2006.01)
CPC C12N 15/8258 (2013.01) [C07K 16/22 (2013.01); C07K 16/241 (2013.01); C12N 9/2402 (2013.01); C12N 15/62 (2013.01); C12N 15/625 (2013.01); C12N 15/8205 (2013.01); C12N 15/8218 (2013.01); C12N 15/8257 (2013.01); C12P 21/005 (2013.01); C12Y 302/01037 (2013.01); C12Y 302/01051 (2013.01); C07K 2317/13 (2013.01); C07K 2317/21 (2013.01); C07K 2317/24 (2013.01); C07K 2319/02 (2013.01); C07K 2319/03 (2013.01); C07K 2319/705 (2013.01)] 19 Claims
 
1. A method for producing a human polypeptide-of-interest with a modified glycosylation pattern, the method comprising introducing into a plant or plant cell:
a nucleic acid sequence encoding the human polypeptide-of-interest translationally fused to a signal peptide for localization in a subcellular compartment;
one or more agents for suppressing expression of a fucosyltransferase and a xylosyltransferase;
at least one polypeptide which confers an agricultural effective trait; and
a nucleic acid sequence encoding an affinity moiety translationally fused to a cell wall binding peptide or a signal peptide for localization in the subcellular compartment,
wherein the affinity moiety is for binding the human polypeptide of interest, and wherein the subcellular compartment is the apoplast or the endoplasmic reticulum.