CPC C12N 15/8258 (2013.01) [C07K 16/22 (2013.01); C07K 16/241 (2013.01); C12N 9/2402 (2013.01); C12N 15/62 (2013.01); C12N 15/625 (2013.01); C12N 15/8205 (2013.01); C12N 15/8218 (2013.01); C12N 15/8257 (2013.01); C12P 21/005 (2013.01); C12Y 302/01037 (2013.01); C12Y 302/01051 (2013.01); C07K 2317/13 (2013.01); C07K 2317/21 (2013.01); C07K 2317/24 (2013.01); C07K 2319/02 (2013.01); C07K 2319/03 (2013.01); C07K 2319/705 (2013.01)] | 19 Claims |
1. A method for producing a human polypeptide-of-interest with a modified glycosylation pattern, the method comprising introducing into a plant or plant cell:
a nucleic acid sequence encoding the human polypeptide-of-interest translationally fused to a signal peptide for localization in a subcellular compartment;
one or more agents for suppressing expression of a fucosyltransferase and a xylosyltransferase;
at least one polypeptide which confers an agricultural effective trait; and
a nucleic acid sequence encoding an affinity moiety translationally fused to a cell wall binding peptide or a signal peptide for localization in the subcellular compartment,
wherein the affinity moiety is for binding the human polypeptide of interest, and wherein the subcellular compartment is the apoplast or the endoplasmic reticulum.
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