CPC C12N 5/0658 (2013.01) [C12N 2501/999 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01)] | 8 Claims |
1. An in vitro method of obtaining myotubes comprising:
(a) differentiating pluripotent stem cells into myogenic progenitors;
(b) terminally differentiating said myogenic progenitors from (a) into mature myotubes in the presence of a combination of DAPT (Da), SB-431542 (S), dexamethasone (De) and forskolin (F); and
(c) isolating said myotubes.
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