US 11,697,798 B2
Enhanced differentiation and maturation of pluripotent stem cell-derived myogenic cells
Rita Perlingeiro, St. Paul, MN (US); Sridhar Selvaraj, Minneapolis, MN (US); and Ricardo Mondragon Gonzalez, Estado de Mexico (MX)
Assigned to Regents of the University of Minnesota, Minneapolis, MN (US)
Appl. No. 16/770,917
Filed by Regents of the University of Minnesota, Minneapolis, MN (US)
PCT Filed Dec. 13, 2018, PCT No. PCT/US2018/065531
§ 371(c)(1), (2) Date Jun. 8, 2020,
PCT Pub. No. WO2019/118768, PCT Pub. Date Jun. 20, 2019.
Claims priority of provisional application 62/598,200, filed on Dec. 13, 2017.
Prior Publication US 2021/0171911 A1, Jun. 10, 2021
Int. Cl. C12N 5/077 (2010.01)
CPC C12N 5/0658 (2013.01) [C12N 2501/999 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01)] 8 Claims
OG exemplary drawing
 
1. An in vitro method of obtaining myotubes comprising:
(a) differentiating pluripotent stem cells into myogenic progenitors;
(b) terminally differentiating said myogenic progenitors from (a) into mature myotubes in the presence of a combination of DAPT (Da), SB-431542 (S), dexamethasone (De) and forskolin (F); and
(c) isolating said myotubes.