US 11,697,793 B2
Compositions and methods of making and using protein-functionalized hydrogels
Amit Pathak, St. Louis, MO (US); and Bapi Sarker, St. Louis, MO (US)
Assigned to Washington University, St. Louis, MO (US)
Filed by Amit Pathak, St. Louis, MO (US); and Bapi Sarker, St. Louis, MO (US)
Filed on Oct. 10, 2019, as Appl. No. 16/598,676.
Claims priority of provisional application 62/743,610, filed on Oct. 10, 2018.
Prior Publication US 2020/0115675 A1, Apr. 16, 2020
Int. Cl. C12N 5/00 (2006.01); C08F 220/58 (2006.01); C08F 220/56 (2006.01); C08F 222/38 (2006.01); G01N 33/48 (2006.01); G01N 33/50 (2006.01); C07C 233/31 (2006.01); G01N 33/483 (2006.01)
CPC C12N 5/0018 (2013.01) [C07C 233/31 (2013.01); C08F 220/56 (2013.01); C08F 220/58 (2013.01); C08F 222/385 (2013.01); G01N 33/4833 (2013.01); G01N 33/5029 (2013.01); C12N 2533/30 (2013.01); C12N 2533/54 (2013.01); C12N 2537/10 (2013.01)] 7 Claims
 
1. A polyacrylamide (PA) hydrogel substrate comprising an aldehyde-containing acrylamide for protein functionalization, wherein protein functionalization of the PA hydrogel enables protein fiber formation of a tunable protein fiber length.