	US 11,697,792 B2
	Effects of space travel on human brain cells
Christopher David Hinojosa, Malden, MA (US); Josiah Sliz, Boston, MA (US); Iosif Pediaditakis, Boston, MA (US); and Sonalee Barthakur, Boston, MA (US)
Assigned to EMULATE, INC., Boston, MA (US)
Filed by EMULATE, INC., Boston, MA (US)
Filed on Dec. 12, 2019, as Appl. No. 16/712,439.
Application 16/712,439 is a continuation of application No. PCT/US2018/037505, filed on Jun. 14, 2018.
Claims priority of provisional application 62/648,715, filed on Mar. 27, 2018.
Claims priority of provisional application 62/561,465, filed on Sep. 21, 2017.
Claims priority of provisional application 62/519,739, filed on Jun. 14, 2017.
Prior Publication US 2020/0181555 A1, Jun. 11, 2020
Int. Cl. C12M 1/00 (2006.01); B01L 3/00 (2006.01); C12M 3/06 (2006.01); G01N 1/18 (2006.01); C12M 1/26 (2006.01)

CPC C12M 29/10 (2013.01) [B01L 3/502738 (2013.01); C12M 23/16 (2013.01); C12M 29/14 (2013.01); B01L 2300/0861 (2013.01); B01L 2400/0605 (2013.01); C12M 33/00 (2013.01); G01N 1/18 (2013.01)]

8 Claims

1. A method of recirculating culture media through microfluidic devices, comprising:

1) providing an assembly, comprising

a) one or more reservoirs comprising cell culture media in fluidic communication with one or more microfluidic devices, said one or more microfluidic devices comprising

i) first and second channels attached mammalian cells on a surface of said first or second channels and

ii) inlet and outlet ports associated with said first and second channels, said inlet and outlet ports in fluidic communication with a recirculation pathway, said cells perfused with fluid; and

2) causing culture media in said one or more reservoirs to flow into said one or more microfluidic devices, thereby causing fluid to exit as effluent from said outlet port of said first or second channels of one or more microfluidic devices, enter said recirculation pathway and directly enter an inlet port of said first or second channel, so that said effluent becomes incoming fluid.