US 11,697,605 B2
Microbial flora analysis system, determination system, microbial flora analysis method, and determination method
Hiroyuki Asako, Osaka (JP); and Fumiyoshi Okazaki, Mie (JP)
Assigned to Sumitomo Chemical Company, Limited, Tokyo (JP)
Appl. No. 15/562,624
Filed by Sumitomo Chemical Company, Limited, Tokyo (JP)
PCT Filed Mar. 30, 2016, PCT No. PCT/JP2016/060523
§ 371(c)(1), (2) Date Sep. 28, 2017,
PCT Pub. No. WO2016/159157, PCT Pub. Date Oct. 6, 2016.
Claims priority of application No. 2015-076946 (JP), filed on Apr. 3, 2015.
Prior Publication US 2018/0105444 A1, Apr. 19, 2018
Int. Cl. C02F 3/00 (2023.01); G16B 40/00 (2019.01); G16B 20/00 (2019.01); G16B 40/20 (2019.01); G16B 20/20 (2019.01); C02F 3/12 (2023.01); C12Q 1/68 (2018.01); C12Q 1/6888 (2018.01)
CPC C02F 3/006 (2013.01) [C02F 3/12 (2013.01); G16B 20/00 (2019.02); G16B 20/20 (2019.02); G16B 40/00 (2019.02); G16B 40/20 (2019.02); C02F 2209/36 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6888 (2013.01); Y02W 10/10 (2015.05)] 8 Claims
 
1. A microbial community analysis method operating a microbial community analysis system, the microbial community analysis method comprising:
generating a plurality of data groups including information indicating a nucleotide sequence of a gene of each of a plurality of microorganisms included in activated sludge in which a water treatment is performed;
calculating a similarity between each data group in the plurality of data groups on the basis of each nucleotide sequence included in the plurality of data groups;
calculating coordinates in a multidimensional space of each of the plurality of data groups on the basis of the calculated similarities;
generating, based on the calculated coordinates, at least one determination rule for determining states of microorganisms from nucleotide sequence data of the gene;
acquiring target information on a plurality of determination target microorganisms included in target activated sludge in which a water treatment is performed from at least one sample obtained from the target activated sludge in which a water treatment is performed, the target information comprising a nucleotide sequence of a gene of each of the plurality of determination target microorganisms, wherein the target activated sludge is undergoing an acclimation;
generating a determination target data group based on the nucleotide sequence of a gene of each of the plurality of determination target microorganisms;
calculating a similarity between the determination target data group and each of the plurality of data groups;
calculating coordinates in a multidimensional space of the determination target data group based on the calculated similarity between the determination target data group and each of the plurality of data groups;
determining at least one state of a plurality of states of the plurality of determination target microorganisms from the calculated coordinates of the determination target data group, wherein the at least one determined state of the plurality of determination target microorganisms is determined based on the at least one determination rule, and the at least one determined state indicates acclimation is completed;
discharging water to be treated into a biological reaction tank containing the target activated sludge in which a water treatment is performed after it is determined that the acclimation is completed.