| CPC B01J 13/04 (2013.01) [B01J 13/125 (2013.01)] | 8 Claims |
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1. A controllable method for preparing phospholipid micelles, comprising:
S1, preparing small phospholipid vesicles:
removing solvent of phospholipid chloroform solution, then fully drying the phospholipid chloroform solution in a vacuum drying oven to completely remove chloroform solvents, adding with deionized water and subjecting to ultrasonic treatment under heating; then preparing small phospholipid vesicle solution with a size of 100 nano-meters (nm) to 200 nm by extruding with a micro extruder;
wherein the phospholipid in the S1 is a dipalmitoyl phosphatidic acid or dipalmitoyl phosphatidylcholine;
the phospholipid in the S1 is in a mass-volume ratio of (1 gram (g) to 2 g):(1 milliliter (mL) to 2 mL) to the deionized water;
S2, preparing a graphene thin-layer electrode substrate:
pressing and sticking a transparent adhesive tape on a surface of a flat layer of a large graphene, and quickly tearing down the tape in parallel to obtain a flat graphene thin layer on the transparent adhesive tape, the graphene thin-layer electrode substrate;
S3, dropwise adding small phospholipid vesicle solution with the size of 100 nm-200 nm and salt solution onto the graphene thin-layer electrode substrate in turn, incubating the substrate at 50-70 degree Celsius (° C.), and washing the substrate to obtain an incubated thin-layer electrode with phospholipid monolayer; and
S4, electroforming phospholipid micelles:
connecting the incubated thin layer electrode with phospholipid monolayer and a platinum wire electrode to electrodes of an electrochemical workstation respectively under a temperature of 50° C.-70° C. and electrochemical potential of −2 Volts (V)-+2 V, followed by reaction for 2 minutes (min)-10 min to obtain phospholipid micelles with different depths and widths on the incubated thin-layer electrode with phospholipid monolayer; maintaining the obtained phospholipid micelles under a stable micellar morphology below 40° C.
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