US 12,352,941 B2
Light sheet microscope with line focus
Heinrich Spiecker, Bielefeld (DE); and Martin Schuette, Bielefeld (DE)
Assigned to Miltenyi Biotec B.V. & Co. KG, Bergisch Gladback (DE)
Appl. No. 17/290,762
Filed by LaVision BioTec GmbH, Bielefeld (DE)
PCT Filed Dec. 10, 2019, PCT No. PCT/EP2019/084324
§ 371(c)(1), (2) Date May 2, 2021,
PCT Pub. No. WO2020/126640, PCT Pub. Date Jun. 25, 2020.
Claims priority of provisional application 62/868,917, filed on Jun. 30, 2019.
Claims priority of provisional application 62/783,231, filed on Dec. 21, 2018.
Prior Publication US 2022/0003979 A1, Jan. 6, 2022
Int. Cl. G02B 21/00 (2006.01); G01N 21/64 (2006.01); G02B 21/26 (2006.01); G02B 21/33 (2006.01); G02B 21/34 (2006.01); G02B 21/36 (2006.01); H04N 23/55 (2023.01)
CPC G02B 21/0032 (2013.01) [G02B 21/0048 (2013.01); G02B 21/006 (2013.01); G02B 21/0072 (2013.01); G02B 21/0076 (2013.01); G02B 21/008 (2013.01); G02B 21/26 (2013.01); G02B 21/33 (2013.01); G02B 21/34 (2013.01); G02B 21/367 (2013.01); H04N 23/55 (2023.01); G01N 21/6458 (2013.01); G01N 2201/1047 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A light sheet microscope for imaging a biological sample, comprising:
at least three beams of collimated light propagating along at least three different propagation axes;
a moveable optical assembly comprising at least three optical subassemblies which focus the at least three beams of light into three line foci forming at least three straight lines, wherein at least one of the three straight lines defines a non-orthogonal angle with respect to its propagation axis and wherein the at least three straight lines are substantially overlapping, and wherein the straight lines and the propagation axes define an excitation plane of the light sheet microscope, and wherein the three substantially overlapping lines are moved laterally in a sequence by moving the movable optical assembly laterally, but wherein the substantially overlapping lines remain in the excitation plane.