	US 12,352,766 B2
	Immunoassay method for free aim in biological sample, and method for detecting NASH in subject
Toru Miyazaki, Tokyo (JP); Takeshi Okanoue, Suita (JP); Tomohide Asai, Tokyo (JP); Yuka Kanetsuki, Tokyo (JP); and Jiro Hirota, Tokyo (JP)
Assigned to SEKISUI MEDICAL CO., LTD., Tokyo (JP); Toru Miyazaki, Tokyo (JP); and SOCIAL WELFARE ORGANIZATION “SAISEIKAI” IMPERIAL GIFT FOUNDATION INC., Tokyo (JP)
Appl. No. 17/427,294
Filed by SEKISUI MEDICAL CO., LTD., Tokyo (JP); Toru Miyazaki, Tokyo (JP); and SOCIAL WELFARE ORGANIZATION “SAISEIKAI” IMPERIAL GIFT FOUNDATION INC., Tokyo (JP)
PCT Filed Jan. 30, 2020, PCT No. PCT/JP2020/003416 § 371(c)(1), (2) Date Jul. 30, 2021, PCT Pub. No. WO2020/158858, PCT Pub. Date Aug. 6, 2020.
Claims priority of application No. 2019-015224 (JP), filed on Jan. 31, 2019.
Prior Publication US 2022/0120762 A1, Apr. 21, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/68 (2006.01); C07K 16/28 (2006.01); G01N 33/53 (2006.01); G01N 33/576 (2006.01)

CPC G01N 33/6893 (2013.01) [C07K 16/2896 (2013.01); G01N 33/5306 (2013.01); G01N 33/576 (2013.01); G01N 2800/085 (2013.01)]

8 Claims

1. An immunoassay method for free apoptosis inhibitor of macrophage (AIM) in a biological sample containing complex AIM and free AIM, the method comprising:

bringing the biological sample into contact with an antibody specifically reacting with free AIM or a fragment thereof having antigen-antibody reaction activity with free AIM in a presence of an added anti-IgM antibody or a fragment thereof having an antigen-antibody reaction activity with IgM, wherein the added anti-IgM antibody or fragment thereof is present in an amount that suppresses non-specific reactions of the antibody specifically reacting with free AIM.