US 12,352,760 B2
Protein scaffold capable of carrying radioactive signals and application in antibody detection
Heng Chen, Jiangsu (CN); Tao Yang, Jiangsu (CN); and Yong Gu, Jiangsu (CN)
Assigned to JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIVERSITY, Jiangsu (CN)
Filed by JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIVERSITY, Jiangsu (CN)
Filed on Sep. 10, 2024, as Appl. No. 18/829,320.
Claims priority of application No. 202311164317.4 (CN), filed on Sep. 11, 2023.
Prior Publication US 2025/0085294 A1, Mar. 13, 2025
Int. Cl. G01N 33/68 (2006.01); C07K 14/00 (2006.01); G01N 33/60 (2006.01)
CPC G01N 33/6854 (2013.01) [C07K 14/001 (2013.01); G01N 33/60 (2013.01)] 2 Claims
OG exemplary drawing
 
1. A detection kit for an anti-butyrophilin 3A1 antibody, comprising:
a radioactive antigen protein AR12A, wherein the radioactive antigen protein AR12A is obtained by labelling the antigen protein AR12A with S35-methionine, and an amino acid sequence of the antigen protein AR12A comprises an amino acid sequence of a protein scaffold and an amino acid sequence of the BTN3A1;
the amino acid sequence of the protein scaffold 
is as follows:
(SEQ ID NO: 12)
IKNILQPGSVDSQTEMVLVNAVYFKGMWEKAFKDEDTQAMPFRMTEQES
 
TPVQMMYQVGSFKVAEMASEKMKILELPYASGELSMLVLLPDDVSGLEE
 
IENAITFEKLTEWTSSSIMEERKIKVYLPRMKMEEKYNLTSVLMALGMT
 
DLF;
the amino acid sequence of the antigen protein AR12A is as follows:
(SEQ ID NO: 19)
LLGGAGYFLWQQQEEKKTQFRKKKREQELREMAWSTMKQEQSTRVKLLE
 
ELRWRSIQYASRGERHSAYNEWKKALFKPADVILDPKTANPILLVSEDQ
 
RSVQRAKEPQDLPDNPERFNWHYCVLGCESFISGRHYWEVEVGDRKEWH
 
IGVCSKNVQRKGWVKMTPENGFWTMGLTDGNKYRTLTEPRTNLKLPKPP
 
KKVGVFLDYETGDISFYNAVDGSHIHTFLDVSFSEALYPVFRILTLEPT
 
ALTICPAPKPSTPPGSSGGGSIKNILQPGSVDSQTEMVLVNAVYFKGMW
 
EKAFKDEDTQAMPFRMTEQESTPVQMMYQVGSFKVAEMASEKMKILELP
 
YASGELSMLVLLPDDVSGLEEIENAITFEKLTEWTSSSIMEERKIKVYL
 
PRMKMEEKYNLTSVLMALGMTDLFPKPSTPPGSSGGGSLLGGAGYFLWQ
 
QQEEKKTQFRKKKREQELREMAWSTMKQEQSTRVKLLEELRWRSIQYAS
 
RGERHSAYNEWKKALFKPADVILDPKTANPILLVSEDQRSVQRAKEPQD
 
LPDNPERFNWHYCVLGCESFISGRHYWEVEVGDRKEWHIGVCSKNVQRK
 
GWVKMTPENGFWTMGLTDGNKYRTLTEPRTNLKLPKPPKKVGVFLDYET
 
GDISFYNAVDGSHIHTFLDVSFSEALYPVFRILTLEPTALTICPA.