US 12,352,757 B2
Pseudo-electropherogram construction from peptide level mass spectrometry data
Andrew Nichols, Redwood City, CA (US); Marshall Bern, San Carlos, CA (US); Yong Joo Kil, San Bruno, CA (US); and Eric Carlson, Cupertino, CA (US)
Assigned to Protein Metrics, LLC, Boston, MA (US)
Filed by Protein Metrics, LLC, Boston, MA (US)
Filed on Jun. 7, 2024, as Appl. No. 18/737,836.
Application 18/737,836 is a continuation of application No. 17/828,465, filed on May 31, 2022, granted, now 12,038,444.
Application 17/828,465 is a continuation of application No. 16/859,758, filed on Apr. 27, 2020, granted, now 11,346,844, issued on May 31, 2022.
Claims priority of provisional application 62/839,507, filed on Apr. 26, 2019.
Prior Publication US 2024/0329052 A1, Oct. 3, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/68 (2006.01); H01J 49/00 (2006.01)
CPC G01N 33/6848 (2013.01) [H01J 49/0036 (2013.01); H01J 49/004 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for generating a pseudo-electropherogram of a large molecule, the method comprising:
receiving mass spectrometry data including mass-to-charge ratio data associated with molecular species that are components of the large molecule, wherein the mass-to-charge ratio data includes mass-to-charge ratio data associated with modified forms of the molecular species;
generating the pseudo-electropherogram based on all or a subset of the modified forms of the molecular species, wherein generating the pseudo-electropherogram comprises:
determining masses of the modified forms of the molecular species based on the mass-to-charge ratio data associated with the modified forms of the molecular species;
matching reference molecular sequences of the large molecule against the determined masses of the of the modified forms of the molecular species to map the modified forms of the molecular species to locations in the large molecule; and
combining calculated charge distributions of the modified forms of the molecular species to generate a distribution of charge variant forms of the large molecule, wherein the calculated charge distributions are calculated based on isoelectric point; and
displaying the pseudo-electropherogram representing different charge variant forms of the large molecule.