US 12,352,753 B2
Kit for measuring measurement target substance and method for measuring measurement target substance
Yoshinori Kanazawa, Ashigarakami-gun (JP); Kazuhei Kaneko, Ashigarakami-gun (JP); and Kouitsu Sasaki, Ashigarakami-gun (JP)
Assigned to FUJIFILM Corporation, Tokyo (JP)
Filed by FUJIFILM Corporation, Tokyo (JP)
Filed on Aug. 4, 2021, as Appl. No. 17/393,578.
Application 17/393,578 is a continuation of application No. PCT/JP2020/004222, filed on Feb. 5, 2020.
Claims priority of application No. 2019-019586 (JP), filed on Feb. 6, 2019.
Prior Publication US 2021/0364524 A1, Nov. 25, 2021
Int. Cl. G01N 33/58 (2006.01); G01N 21/64 (2006.01)
CPC G01N 33/582 (2013.01) [G01N 21/6428 (2013.01); G01N 33/587 (2013.01); G01N 2021/6439 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A kit for measuring a measurement target substance in a biological sample, the kit comprising:
a luminescently labeled particle that has a first binding substance having a binding property to a measurement target substance;
a substrate that has a detection area on a metal film having a second binding substance having a binding property to any one of the measurement target substance or the first binding substance; and
a non-luminescent high molecular particle that contains a structural unit represented by Formula (A) or Formula (B),

OG Complex Work Unit Chemistry
in the formula, R51 represents a hydrogen atom or a methyl group, X51 represents an oxygen atom or NR53, and R52 represents a substituted or unsubstituted alkyl group, a substituted or unsubstituted aryl group, or a substituted or unsubstituted heteroaryl group, where R53 represents a hydrogen atom, a substituted or unsubstituted alkyl group, a substituted or unsubstituted aryl group, or a substituted or unsubstituted heteroaryl group, and

OG Complex Work Unit Chemistry
in the formula, R54 and R55 each independently represent a hydrogen atom or a methyl group, X52 and X53 each independently represent an oxygen atom or NR56, and L represents a substituted or unsubstituted polyalkyleneoxy chain, a substituted or unsubstituted alkylene group, a substituted or unsubstituted arylene group, or a substituted or unsubstituted heteroarylene group, where R56 represents a hydrogen atom, a substituted or unsubstituted alkyl group, a substituted or unsubstituted aryl group, or a substituted or unsubstituted heteroaryl group,
wherein the luminescently labeled particle is a particle that contains a fluorescent dye represented by Formula (1) or a fluorescent dye represented by Formula (10),

OG Complex Work Unit Chemistry
in the formula, X represents CR67 or N,
R61 to R67 represents a hydrogen atom, an alkyl group, a cycloalkyl group, an aliphatic heterocyclic group, an alkenyl group, a cycloalkenyl group, an alkynyl group, a hydroxyl group, a mercapto group, an alkoxy group, an alkylthio group, an aryloxy group, an arylthio group, an aryl group, a heteroaryl group, a halogen atom, a cyano group, a formyl group, an R—CO-group, a carboxy group, an R—O—CO-group, an R—CO—O-group, an (RA)2N—CO-group, an amino group, a nitro group, or a silyl group, which may further have a substituent,
where R represents an alkyl group, a cycloalkyl group, an aliphatic heterocyclic group, an alkenyl group, a cycloalkenyl group, an alkynyl group, an aryl group, or a heteroaryl group, and RA represents a hydrogen atom, an alkyl group, a cycloalkyl group, an aliphatic heterocyclic group, an alkenyl group, a cycloalkenyl group, an alkynyl group, an aryl group, or a heteroaryl group, and
R68 and R69 represent an alkyl group, a cycloalkyl group, an aliphatic heterocyclic group, an alkenyl group, a cycloalkenyl group, an alkynyl group, a hydroxyl group, a mercapto group, an alkoxy group, an alkylthio group, an aryloxy group, an arylthio group, an aryl group, a heteroaryl group, or a halogen atom, which may further have a substituent, and

OG Complex Work Unit Chemistry
in the formula, m1 and m2 each independently represent an integer of 0 to 4, and any one of m1 or m2 is at least one, M represents a metalloid atom or a metal atom, R1, R2, and R3 each independently represent a hydrogen atom, an alkyl group, an aryl group, a heterocyclic group, an ethenyl group, an ethynyl group, an acyl group, an alkoxy group, an aryloxy group, an alkylthio group, or an arylthio group, which may have a substituent, Y1 and Y2 each independently represent a halogen atom, an alkyl group, an aryl group, a heterocyclic group, a hydroxy group, an alkoxy group, an aryloxy group, an alkylthio group, an arylthio group, an ethenyl group, or an ethynyl group, which may have a substituent, and Y1 and Y2 may be linked to each other to form a ring, Ar11 and Ar12 each independently represent an aromatic ring which may have a substituent, Z1 and Z2 each independently represent an aryl group, a heterocyclic group, or an amino group, which may have a substituent, and in a case where m1 is two or more, a plurality of Z1's may be the same group or groups different from each other, and in a case where m2 is two or more, a plurality of Z2's may be the same group or groups different from each other,
wherein the non-luminescent high molecular particle is a latex obtained by homogeneously suspending a synthetic polymer,
wherein the non-luminescent high molecular particle contains a styrene unit, a unit of acrylic acid or a salt thereof, or a unit of methacrylic acid or a salt thereof, as a structural unit other than the structural unit represented by Formula (A) or Formula (B), and
a core particle of the non-luminescent high molecular particle consists of a homopolymer of styrene, and the structural unit represented by Formula (A) or Formula (B) is present on the surface of the particles.