US 12,352,747 B2
System for analyzing quantitative lateral flow chromatography
Franz Paul Armbruster, Bensheim (DE); Felix Walzer, Bensheim (DE); and Ben John, Bensheim (DE)
Assigned to Immundiagnostik AG, Bensheim (DE)
Appl. No. 17/052,375
Filed by Immundiagnostik AG, Bensheim (DE)
PCT Filed May 7, 2019, PCT No. PCT/EP2019/061749
§ 371(c)(1), (2) Date Nov. 2, 2020,
PCT Pub. No. WO2019/215199, PCT Pub. Date Nov. 14, 2019.
Claims priority of application No. 10 2018 110 861.3 (DE), filed on May 7, 2018.
Prior Publication US 2021/0172945 A1, Jun. 10, 2021
Int. Cl. G01N 33/558 (2006.01); G01N 15/06 (2006.01); G01N 21/84 (2006.01); G01N 33/543 (2006.01); G01N 15/01 (2024.01)
CPC G01N 33/54388 (2021.08) [G01N 15/0625 (2013.01); G01N 21/8483 (2013.01); G01N 15/01 (2024.01); G01N 2015/0687 (2013.01)] 11 Claims
 
1. A system for determining the presence and content of an analyte in a test sample subjected to lateral flow chromatography, the system comprising
a test device adapted to house a lateral flow test, wherein said test device further displays one or more reference images and a region of interest of said lateral flow test which bears one or more visible response zones indicating the presence and content of said analyte in said test sample and a control zone, and
a portable processor device comprising a digital camera, a source of light and a processor, wherein said processor is configured to process transient digital images of a video captured by said camera and to represent an analytical result, wherein:
said processor is configured to analyze sequentially a plurality of the transient digital images of the video for the presence of one or more permanent reference images;
said processor is configured upon finding one or more permanent reference images, to determine the distance between the found permanent reference image and the digital camera to determine if the distance is acceptable;
said processor is configured to analyze each transient digital image of the video having said permanent reference image at an acceptable distance for said region of interest; and
said processor is configured to examine each of said regions of interest of each transient digital image of the video having a permanent reference image at an acceptable distance for its measures of light reflected and, if the light reflected is in line with predefined measures, said processor is configured to retrieve and save said transient digital image so that only digital images of the region of interest will be processed and analyzed for the optical intensities of said zones which are based on predefined light conditions.