US 12,352,746 B2
Peptide-comprising electrode
Eduardo Maffud Cilli, Araraquara (BR); and Paulo Roberto Bueno, Araraquara (BR)
Assigned to Oxford University Innovation Limited, Oxford (GB); and Universidade Estadual Paulista “Júlio De Mequita Filho”—UNESP, Sao Paulo (BR)
Appl. No. 16/962,958
Filed by Oxford University Innovation Limited, Oxford (GB); and Universidade Estadual Paulista “Júlio De Mesquita Filho”—UNESP, São Paulo (BR)
PCT Filed Jan. 23, 2019, PCT No. PCT/GB2019/050183
§ 371(c)(1), (2) Date Jul. 17, 2020,
PCT Pub. No. WO2019/145706, PCT Pub. Date Aug. 1, 2019.
Claims priority of application No. 1801074 (GB), filed on Jan. 23, 2018; and application No. 1813869 (GB), filed on Aug. 24, 2018.
Prior Publication US 2021/0132050 A1, May 6, 2021
Int. Cl. G01N 33/543 (2006.01); C07K 5/11 (2006.01); C07K 5/113 (2006.01); C07K 7/06 (2006.01); G01N 27/02 (2006.01); G01N 27/327 (2006.01)
CPC G01N 33/5438 (2013.01) [C07K 5/1019 (2013.01); C07K 5/1021 (2013.01); C07K 7/06 (2013.01); G01N 27/026 (2013.01); G01N 27/3276 (2013.01)] 21 Claims
OG exemplary drawing
 
1. An electrode for use in electrochemical sensing of a target species, which electrode comprises:
(i) an electrically conductive substrate;
(ii) a peptide monolayer comprising a plurality of peptide molecules that are each:
(a) disposed on the substrate;
(b) attached to a redox active species; and
(c) attached to a receptor that is capable of binding to the target species;
wherein:
the peptide molecules are each from three to five amino acid residues in length, including a first terminal amino acid residue that is adjacent to the substrate and a second terminal amino acid; and
the redox active species and the receptor are each attached to the second terminal amino acid;
the attachment to one of the redox active species and the receptor is via a free amine or carboxylic acid group of the second terminal amino acid, and the attachment of the other of the redox active species and the receptor is via a reactive side group present in the second terminal amino acid; and
in each peptide molecule one of the first terminal amino acid residue and the second terminal amino acid is a C-terminus of the peptide molecule and the other of the first terminal amino acid residue and the second terminal amino acid is an N-terminus of the peptide molecule.