US 12,352,720 B2
Redox probing for chemical information
Eunkyoung Kim, Woodstock, MD (US); Gregory F. Payne, Hunt Valley, MD (US); Mijeong Kang, Rockville, MD (US); Reza Ghodssi, Potomac, MD (US); Thomas E. Winkler, Greenbelt, MD (US); George Banis, Silver Spring, MD (US); Christopher Kitchen, Ellicott City, MD (US); Deanna L. Kelly, York, PA (US); and William E. Bentley, Annapolis, MD (US)
Assigned to UNIVERSITY OF MARYLAND, COLLEGE PARK, College Park, MD (US); and University of Maryland, Baltimore, Baltimore, MD (US)
Appl. No. 16/465,243
Filed by University of Maryland, College Park, College Park, MD (US); and University of Maryland, Baltimore, Baltimore, MD (US)
PCT Filed Dec. 4, 2017, PCT No. PCT/US2017/064485
§ 371(c)(1), (2) Date May 30, 2019,
PCT Pub. No. WO2018/102808, PCT Pub. Date Jun. 7, 2018.
Claims priority of provisional application 62/526,576, filed on Jun. 29, 2017.
Claims priority of provisional application 62/429,610, filed on Dec. 2, 2016.
Prior Publication US 2019/0346400 A1, Nov. 14, 2019
Int. Cl. G01N 27/327 (2006.01); G01N 33/49 (2006.01)
CPC G01N 27/3277 (2013.01) [G01N 33/49 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method for determining a redox-based condition associated with oxidative stress, using a measuring device, the method comprising:
contacting a sample from a patient at a point-of-care with one or more redox-mediators defining a redox potential;
providing an electrical input to the sample, the electrical input being a sequence having:
a first input pulse defined by a first oxidative voltage greater than the redox potential and imposed for a first duration; and
at least a second input pulse defined by a second oxidative voltage equal to the first oxidative voltage and imposed for a second duration different than the first duration, the first input pulse and the at least second input pulse being separated by an off voltage, the off voltage being imposed for a duration greater than the first duration;
measuring by electrodes a dynamic pattern of current and charge over a period of time of the sample based on the one or more redox-mediators and the electrical input;
measuring by an optical sensor an optical output over the period of time of the sample based on the one or more redox-mediators and the electrical input; and
using the dynamic pattern of current and charge and the optical output over the period of time to derive a plurality of electrochemical or spectroelectrochemical pattern signal signatures for determining the redox-based condition, thereby assaying for a presence of the redox-based condition associated with oxidative stress within the patient at the point-of-care.