US 12,352,681 B2
Apparatus and methods for sample acquisition
Van Chandler, Austin, TX (US); and Joaquin Campos, Austin, TX (US)
Assigned to CHANDLERTEC INC., Austin, TX (US)
Filed by CHANDLERTEC INC., Austin, TX (US)
Filed on Nov. 20, 2023, as Appl. No. 18/514,204.
Application 17/386,742 is a division of application No. 16/235,076, filed on Dec. 28, 2018, granted, now 11,099,117, issued on Aug. 24, 2021.
Application 18/514,204 is a continuation of application No. 17/386,742, filed on Jul. 28, 2021, abandoned.
Claims priority of provisional application 62/611,220, filed on Dec. 28, 2017.
Prior Publication US 2024/0248022 A1, Jul. 25, 2024
Int. Cl. G01N 15/14 (2024.01); C12Q 1/68 (2018.01); G01N 15/1404 (2024.01); G01N 15/1409 (2024.01); G01N 15/149 (2024.01)
CPC G01N 15/1425 (2013.01) [C12Q 1/68 (2013.01); G01N 15/1404 (2013.01); G01N 15/1409 (2024.01); G01N 2015/1415 (2013.01); G01N 15/149 (2024.01)] 5 Claims
OG exemplary drawing
 
1. A method of obtaining a plurality of samples for flow cytometry analysis, the method comprising:
positioning a sample acquisition apparatus above a well plate comprising a plurality of wells; and
sequentially directing a flow of gas from the sample acquisition apparatus into each well in the plurality of wells, wherein:
the flow of gas from the sample acquisition apparatus into each well displaces a portion of a sample from each well in the plurality of wells into the sample acquisition apparatus; and
the sample acquisition apparatus does not move relative to the well plate, wherein:
each portion of the sample from each well in the plurality of wells directed into the sample acquisition apparatus is separated by a volume of gas from the flow of gas; and
the sample acquisition apparatus comprises:
a stack of plates;
a plurality of seals coupled to a first plate of the stack of plates, wherein each seal of the plurality of seals is configured to seal to a well in a well plate;
a first plurality of conduits extending through the stack of plates and the plurality of seals;
a plurality of gas valves, wherein each valve is in fluid communication with a conduit in the first plurality of conduits;
a sample port in a second plate of the stack of plates; and
a second plurality of conduits extending through the plurality of seals and the stack of plates, wherein the second plurality of conduits is in fluid communication with the sample port.