US 12,351,869 B1
Nucleic acid sequencing compositions and methods
Harlan S. Robins, Seattle, WA (US); and Jason H. Bielas, Seattle, WA (US)
Assigned to Digital Biotechnologies, Inc., Seattle, WA (US)
Filed by Digital Biotechnologies, Inc., Seattle, WA (US)
Filed on Nov. 18, 2021, as Appl. No. 17/455,656.
Claims priority of provisional application 63/250,933, filed on Sep. 30, 2021.
Claims priority of provisional application 63/116,072, filed on Nov. 19, 2020.
Int. Cl. C12Q 1/6874 (2018.01); G16B 30/00 (2019.01)
CPC C12Q 1/6874 (2013.01) [G16B 30/00 (2019.02)] 18 Claims
OG exemplary drawing
 
1. A synthetic strand for nucleic acid sequencing, comprising:
a plurality of rotatable molecular disks, wherein the plurality of rotatable molecular disks comprises molecular disks each comprising:
a first moiety that binds to adenine (A);
a second moiety that binds to cytosine (C);
a third moiety that binds to guanine (G);
a fourth moiety that binds to thymine (T), uracil (U), or both (T/U); and
a position indicator that indicates the rotational position of the molecular disk, wherein the first, second, third, and fourth moieties are spaced about the perimeter of the molecular disk, and
wherein the molecular disks are sized and spaced along one or more base strands to enable hybridization of the synthetic strand to a nucleic acid.