	US 12,351,851 B2
	Disruption of CDC42 effectors in yeast for increased alcohol and lysine production
Jaclyn Diana Demartini, Palo Alto, CA (US); and Celia Emily Gaby Payen, Wilmington, DE (US)
Assigned to DANISCO US INC., Palo Alto, CA (US)
Appl. No. 17/621,957
Filed by DANISCO US INC, Palo Alto, CA (US)
PCT Filed Jun. 22, 2020, PCT No. PCT/US2020/038910 § 371(c)(1), (2) Date Dec. 22, 2021, PCT Pub. No. WO2020/263732, PCT Pub. Date Dec. 30, 2020.
Claims priority of provisional application 62/865,448, filed on Jun. 24, 2019.
Prior Publication US 2022/0251608 A1, Aug. 11, 2022
Int. Cl. C12P 7/06 (2006.01); C10L 1/02 (2006.01); C12N 1/18 (2006.01); C12N 15/52 (2006.01); C12N 15/81 (2006.01); C12P 13/08 (2006.01)

CPC C12P 7/06 (2013.01) [C10L 1/02 (2013.01); C12N 1/18 (2013.01); C12N 15/52 (2013.01); C12N 15/81 (2013.01); C12P 13/08 (2013.01); C12Y 102/0101 (2013.01); C12Y 203/01008 (2013.01); C12Y 401/02009 (2013.01); C10L 2200/0469 (2013.01); C10L 2290/26 (2013.01); C12N 2800/102 (2013.01)]

13 Claims

1. Modified yeast cells derived from parental yeast cells, the modified yeast cells comprising:

(a) a genetic alteration that causes the modified yeast cells to produce a decreased amount of functional Cdc42 effector polypeptides compared to the parental yeast cells, wherein the genetic alteration comprises a disruption of a YHR061c gene, or homolog, thereof, encoding a Gic1 polypeptide and/or disruption of a YDR309c gene, or homolog, thereof, encoding a Gic2 polypeptide, present in the parental yeast cells; and

(b) an exogenous gene encoding a carbohydrate processing enzyme;

wherein the modified yeast cells demonstrate increased alcohol production and/or increased lysine production compared to the parental yeast cells under equivalent fermentation conditions.