US 12,351,812 B2
Recombinant oncolytic virus
Ming Luo, Atlanta, GA (US)
Assigned to Georgia State University Research Foundation, Inc., Atlanta, GA (US)
Appl. No. 16/614,441
Filed by Georgia State University Research Foundation, Inc., Atlanta, GA (US)
PCT Filed May 16, 2018, PCT No. PCT/US2018/032915
§ 371(c)(1), (2) Date Nov. 18, 2019,
PCT Pub. No. WO2018/213412, PCT Pub. Date Nov. 22, 2018.
Claims priority of provisional application 62/508,430, filed on May 19, 2017.
Prior Publication US 2020/0199624 A1, Jun. 25, 2020
Int. Cl. A61K 39/385 (2006.01); A61K 35/766 (2015.01); C07K 14/47 (2006.01); C12N 7/00 (2006.01); C12N 15/86 (2006.01)
CPC C12N 15/86 (2013.01) [A61K 35/766 (2013.01); C07K 14/4702 (2013.01); C12N 7/00 (2013.01); C12N 2310/141 (2013.01); C12N 2760/20021 (2013.01); C12N 2760/20032 (2013.01); C12N 2760/20043 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A recombinant viral vector comprising a nucleic acid encoding a Second Mitochondria-derived Activator of Caspases (SMAC) protein inserted within the genome of a vesicular stomatitis virus (VSV),
wherein the SMAC protein consists of the amino acid sequence SEQ ID NO:1 or 2, or a conservative variant thereof having an IAP-binding motif comprising the amino acid sequence SEQ ID NO:3 and that specifically binds to at least a portion of an Inhibitor of Apoptosis Protein (IAP),
wherein the virus genome comprises at least a nucleoprotein (N or NP) gene, a phosphoprotein (P) gene, a glycoprotein (G) gene, and a matrix protein (M) gene,
wherein the G gene encodes a modified G protein that consists of the amino acid sequence SEQ ID NO:5 with a peptide sequence six amino acids or less comprising an RGD sequence inserted after amino acid position 190 of SEQ ID NO:5, and
wherein the nucleic acid encoding SMAC protein is inserted between the M gene and glycoprotein (G) gene.