	US 12,351,647 B2
	Binding agent and assay for PIVKA
Bernhard Eckert, Weilheim (DE); Michael Gerg, Munich (DE); Johann Karl, Peissenberg (DE); Martin Kaufmann, Weilheim (DE); Julia Riedlinger, Ottobrunn (DE); Magdelena Swiatek-de-Lange, Penzberg (DE); Lars Hillringhaus, Koenigsdorf-Schoerain (DE); Klaus Hirzel, Baierbrunn (DE); and Marcus-Rene Lisy, Geretsried (DE)
Assigned to Roche Diagnostics Operations, Inc., Indianapolis, IN (US)
Filed by Roche Diagnostics Operations, Inc., Indianapolis, IN (US)
Filed on Aug. 24, 2022, as Appl. No. 17/821,937.
Application 17/821,937 is a continuation of application No. 16/740,778, filed on Jan. 13, 2020, granted, now 11,466,098.
Application 16/740,778 is a continuation of application No. PCT/EP2018/068871, filed on Jul. 12, 2018.
Claims priority of application No. 17181242 (EP), filed on Jul. 13, 2017.
Prior Publication US 2023/0055005 A1, Feb. 23, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 16/36 (2006.01); G01N 33/574 (2006.01); G01N 33/86 (2006.01)

CPC C07K 16/36 (2013.01) [G01N 33/57438 (2013.01); G01N 33/86 (2013.01); C07K 2317/565 (2013.01); G01N 2333/745 (2013.01)]

20 Claims

1. A method for the production of an antibody, the method comprising:

a) selecting the antibody to be produced, wherein the antibody is selected by:

(i) incubating a candidate antibody with a synthetic peptide of SEQ ID NO:1 and measuring their reactivity;

(ii) incubating the candidate antibody with a synthetic peptide of SEQ ID NO:2 and measuring their reactivity;

(iii) incubating the candidate antibody with a synthetic peptide of SEQ ID NO:3 and measuring their reactivity;

(iv) comparing the reactivity of the candidate antibody with the synthetic peptide of SEQ ID NO:1 and the reactivity of the candidate antibody with the synthetic peptide of SEQ ID NO:2;

(v) comparing the reactivity of the candidate antibody with the synthetic peptide of SEQ ID NO:1 and the reactivity of the candidate antibody with the synthetic peptide of SEQ ID NO:3; and

(vi) selecting the candidate antibody as the antibody of the method if the candidate antibody binds to a synthetic peptide of SEQ ID NO: 1, has at least a 10-fold binding preference for the peptide of SEQ ID NO:1 as compared to a synthetic peptide of SEQ ID NO:2, and binds to a synthetic peptide of SEQ ID NO:3 at least as well as compared to the peptide of SEQ ID NO:1;

b) culturing a host cell comprising one or more vectors, the one or more vectors comprising one or more nucleic acid molecules encoding the antibody, and

c) isolating the antibody produced.