US 12,351,630 B2
PD1 and/or LAG3 binders
Edward Bowman, Redwood City, CA (US); Maribel Beaumont, San Mateo, CA (US); Marie-Ange Buyse, Merelbeke (BE); Carlo Boutton, Heusden (BE); Bruno Dombrecht, Heusden (BE); Robert A. Kastelein, Portola Valley, CA (US); and David Vlerick, Gentbrugge (BE)
Assigned to Merck Sharp & Dohme LLC, Rahway, NJ (US)
Filed by Merck Sharp & Dohme LLC, Rahway, NJ (US)
Filed on Oct. 8, 2021, as Appl. No. 17/497,597.
Application 16/415,530 is a division of application No. 15/353,919, filed on Nov. 17, 2016, granted, now 10,323,090, issued on Oct. 26, 2021.
Application 17/497,597 is a continuation of application No. 16/415,530, filed on May 17, 2019, granted, now 11,155,619.
Claims priority of provisional application 62/257,009, filed on Nov. 18, 2015.
Prior Publication US 2022/0041727 A1, Feb. 10, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 16/28 (2006.01); A61K 39/395 (2006.01); A61K 45/06 (2006.01); C07K 16/18 (2006.01); C07K 16/46 (2006.01)
CPC C07K 16/2803 (2013.01) [A61K 39/3955 (2013.01); A61K 45/06 (2013.01); C07K 16/18 (2013.01); C07K 16/2818 (2013.01); C07K 16/468 (2013.01); C07K 2317/22 (2013.01); C07K 2317/24 (2013.01); C07K 2317/31 (2013.01); C07K 2317/33 (2013.01); C07K 2317/34 (2013.01); C07K 2317/565 (2013.01); C07K 2317/569 (2013.01); C07K 2317/62 (2013.01); C07K 2317/76 (2013.01); C07K 2317/92 (2013.01)] 7 Claims
 
1. A LAG3 binder comprising an immunoglobulin single variable domain (ISVD) that binds to LAG3 comprising
a CDR1 comprising the amino acid sequence GRTFSDYVMG (SEQ ID NO: 65) or DYVMG (amino acids 6-10 of SEQ ID NO: 65);
a CDR2 comprising the amino acid sequence AISESGGRTHYADSVKG (SEQ ID NO: 66) or AISESGGRTH (amino acids 1-10 of SEQ ID NO: 66); and
a CDR3 comprising the amino acid sequence TLLWWTSEYAPIKANDYDY (SEQ ID NO: 67); wherein the LAG3 binder binds human LAG3 and wherein the ISVD further comprises a substitution of the amino acids at positions 11 and 89 with amino acids V and L, respectively, wherein the numbering is according to Kabat.