US 12,351,613 B2
Production of glycoproteins using manganese
Christopher Kenyon Crowell, Thousand Oaks, CA (US); and Gustavo Enrique Grampp, Thousand Oaks, CA (US)
Assigned to AMGEN INC., Thousand Oaks, CA (US)
Filed by AMGEN INC., Thousand Oaks, CA (US)
Filed on Aug. 31, 2022, as Appl. No. 17/823,883.
Application 17/823,883 is a continuation of application No. 16/947,265, filed on Jul. 24, 2020, abandoned.
Application 16/947,265 is a continuation of application No. 15/683,521, filed on Aug. 22, 2017, abandoned.
Application 15/683,521 is a continuation of application No. 15/004,716, filed on Jan. 22, 2016, abandoned.
Application 15/004,716 is a continuation of application No. 14/087,747, filed on Nov. 22, 2013, granted, now 9,273,110.
Application 14/087,747 is a continuation of application No. 13/112,940, filed on May 20, 2011, granted, now 8,617,878.
Application 13/112,940 is a continuation of application No. 11/634,506, filed on Dec. 6, 2006, granted, now 7,972,810.
Claims priority of provisional application 60/748,880, filed on Dec. 8, 2005.
Prior Publication US 2022/0402986 A1, Dec. 22, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/00 (2006.01); C07K 14/505 (2006.01); C07K 14/575 (2006.01); C12N 5/00 (2006.01); C12P 21/00 (2006.01)
CPC C07K 14/505 (2013.01) [C07K 14/575 (2013.01); C12N 5/0018 (2013.01); C12P 21/005 (2013.01); C12N 2500/20 (2013.01); C12N 2500/32 (2013.01); C12N 2510/02 (2013.01)] 18 Claims
 
1. A method for producing an erythropoietic composition comprising sialylated erythropoiesis-stimulating glycoproteins, comprising the steps of:
(a) growing a manganese-responsive CHO host cell which produces recombinant erythropoiesis-stimulating glycoproteins in a culture medium containing a concentration of manganese from 0.4 to 40 μM, wherein the erythropoiesis-stimulating glycoproteins comprise a mature protein comprising amino acids 1-165 of SEQ ID NO: 2 or amino acids 1-165 of SEQ ID NO: 3; and
(b) recovering an erythropoietic composition wherein less than 5% of the erythropoiesis-stimulating glycoproteins are lower sialylated.