	US 12,351,608 B2
	Methods of separating phosvitin and HDL from an egg yolk product and resulting compositions
William Perrin, White Rock (CA); Christopher Nichols, Coquitlam (CA); Harry Ten Haaf, Langley (CA); and Glenn Nichols, Maple Ridge (CA)
Assigned to Ecovatec Solutions Inc., Abbotsford (CA)
Appl. No. 17/311,664
Filed by Ecovatec Solutions Inc., Abbotsford (CA)
PCT Filed Dec. 5, 2019, PCT No. PCT/CA2019/051754 § 371(c)(1), (2) Date Jun. 7, 2021, PCT Pub. No. WO2020/113341, PCT Pub. Date Jun. 11, 2020.
Claims priority of application No. CA 3026673 (CA), filed on Dec. 6, 2018.
Prior Publication US 2022/0064232 A1, Mar. 3, 2022
Int. Cl. C07K 14/465 (2006.01); A23J 1/08 (2006.01); A23J 3/04 (2006.01); A23J 3/34 (2006.01); A23L 15/00 (2016.01); A23L 33/18 (2016.01); A61K 8/98 (2006.01); A61K 38/00 (2006.01); C07K 1/14 (2006.01)

CPC C07K 14/465 (2013.01) [A23J 1/08 (2013.01); A23J 3/04 (2013.01); A23J 3/341 (2013.01); A23L 15/25 (2016.08); A23L 33/18 (2016.08); A61K 8/982 (2013.01); C07K 1/14 (2013.01); A23V 2002/00 (2013.01); A61K 38/00 (2013.01)]

18 Claims

1. A method of separating phosvitin and high-density lipoprotein (HDL) proteins from an egg yolk composition, the egg yolk composition including HDL proteins bound to phosvitin, the method comprising:

selectively hydrolysing at least a portion of the HDL proteins using one or more proteolytic enzymes to cause the HDL proteins and phosvitin to become unbound and forming a hydrolysed solution comprising hydrolysed HDL, phosvitin and peptides, in which at least a portion of the phosvitin in the egg yolk composition is both: a) unbound from the HDL proteins, and b) remains fully unhydrolysed; and

separating the hydrolysed HDL from the phosvitin and peptides to form a separated hydrolysed HDL composition and a separated phosvitin and peptide solution.