Compositions and related methods for modulating transcriptional activation by incorporating gag motifs upstream of core promoter elements
Michael Paul Timko, Charlottesville, VA (US); Paul J. Rushton, Brookings, SD (US); and Marta Tatiana Bokowiec, Charlottesville, VA (US)
Assigned to University Of Virginia Patent Foundation, Charlottesville, VA (US)
Filed by UNIVERSITY OF VIRGINIA PATENT FOUNDATION, Charlottesville, VA (US)
Filed on Dec. 23, 2019, as Appl. No. 16/725,065.
Application 16/725,065 is a division of application No. 15/618,578, filed on Jun. 9, 2017, granted, now 10,550,162.
Application 15/618,578 is a continuation of application No. 14/658,529, filed on Mar. 16, 2015, granted, now 9,677,083, issued on Jun. 13, 2017.
Application 14/658,529 is a continuation of application No. 12/676,867, granted, now 8,980,633, issued on Mar. 17, 2015, previously published as PCT/US2008/010446, filed on Sep. 5, 2008.
Claims priority of provisional application 60/935,947, filed on Sep. 7, 2007.
Claims priority of provisional application 60/935,948, filed on Sep. 7, 2007.
Prior Publication US 2020/0123211 A1, Apr. 23, 2020
1. A plant comprising an expression vector comprising four copies of a tripartite G-box like element/AT-rich element/GCC-like box element (GAG) motif operably linked to a heterologous promoter, wherein the G-box like element comprises the nucleic acid sequence of SEQ ID NO: 8, wherein the AT-rich element comprises the nucleic acid sequence of SEQ ID NO: 11, wherein the GCC-like box element comprises the nucleic acid sequence of SEQ ID NO: 7, wherein the four copies of the tripartite GAG motif are capable of inducing the heterologous promoter in the presence of methyl jasmonate, and wherein the promoter is operably linked to (a) a polynucleotide encoding a polypeptide or (b) a polynucleotide encoding an inhibitory nucleic acid molecule.