US 12,350,369 B2
Serial filtration to generate small cholesterol-containing liposomes
Stephen Morton, Mountain View, CA (US); and Zach Scott, Santa Cruz, CA (US)
Assigned to Verily Life Sciences LLC, Dallas, TX (US)
Filed by Verily Life Sciences LLC, South San Francisco, CA (US)
Filed on Dec. 14, 2021, as Appl. No. 17/550,935.
Application 17/550,935 is a continuation of application No. 16/314,208, granted, now 11,246,832, previously published as PCT/US2017/039777, filed on Jun. 28, 2017.
Claims priority of provisional application 62/355,552, filed on Jun. 28, 2016.
Prior Publication US 2022/0105037 A1, Apr. 7, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 9/127 (2025.01); A61K 9/1277 (2025.01); B01D 61/58 (2006.01)
CPC A61K 9/1277 (2013.01) [A61K 9/127 (2013.01); B01D 61/58 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A system for producing liposomes from a lipid suspension, comprising:
a filter housing having an inlet and an outlet;
a filter assembly disposed inside the filter housing, the filter assembly comprising:
two or more filters disposed in series;
a panel comprising an orifice disposed between a pair of adjacent filters, wherein an outer surface of the panel surrounding the central orifice is configured to constrict the flow of a lipid suspension between the adjacent filters through the central orifice;
wherein the orifice has a diameter that is less than an average diameter of the two or more filters; and
wherein liposomes produced by passing the lipid suspension through the system at a pressure in the filter assembly less than 300 psi, are smaller in diameter and more monodisperse than liposomes produced with the same number of filters not in series.