	US 11,692,200 B2
	Method for improving rice yield and/or rice blast resistance and protein used thereof
Li Li, Changsha (CN); Yixing Li, Changsha (CN); Dabing Zhang, Changsha (CN); Mudan Qiu, Changsha (CN); Tiankang Wang, Changsha (CN); and Shufeng Song, Changsha (CN)
Assigned to HUNAN HYBRID RICE RESEARCH CENTER, Changsha (CN)
Appl. No. 17/427,570
Filed by HUNAN HYBRID RICE RESEARCH CENTER, Changsha (CN)
PCT Filed Jan. 21, 2020, PCT No. PCT/CN2020/073414 § 371(c)(1), (2) Date Jul. 30, 2021, PCT Pub. No. WO2020/156367, PCT Pub. Date Aug. 6, 2020.
Claims priority of application No. 201910107261.6 (CN), filed on Feb. 2, 2019; and application No. 201910107277.7 (CN), filed on Feb. 2, 2019.
Prior Publication US 2022/0106605 A1, Apr. 7, 2022
Int. Cl. C12N 15/82 (2006.01)

CPC C12N 15/8261 (2013.01) [C12N 15/8213 (2013.01); C12N 15/8282 (2013.01)]

5 Claims

1. A method for producing a rice variety, the method comprising:

transforming a sgRNA expression vector into an original rice variety using a CRISPR/Cas9 method to produce a target rice variety,

wherein the sgRNA expression vector comprises a target sequence as set forth in SEQ ID NO: 8,

wherein the transformation causes a loss of function in a gene encoding the RAY1 protein of SEQ ID NO:1;

wherein said gene has the polynucleotide sequence of SEQ ID NO:2 or SEQ ID NO:3; and

wherein the target rice variety exhibits an increase in an attribute selected from the group consisting of yield, grain size, resistance to rice blast, height, and internode lengthening relative to the original rice variety.