US 11,054,422 B2
Circulating astrocytes and MFSD2A as biomarkers
Sheng-He Huang, Arcadia, CA (US)
Assigned to Sheng-He Huang, Arcadia, CA (US); and Alec Chan, San Gabriel, CA (US)
Filed by Sheng-He Huang, Arcadia, CA (US); and Alec Chan, San Gabriel, CA (US)
Filed on Jun. 4, 2019, as Appl. No. 16/431,559.
Application 16/431,559 is a division of application No. 15/436,859, filed on Feb. 19, 2017, granted, now 10,345,302.
Prior Publication US 2019/0293647 A1, Sep. 26, 2019
Int. Cl. G01N 33/569 (2006.01); A61K 45/00 (2006.01); A61K 31/13 (2006.01)
CPC G01N 33/56966 (2013.01) [A61K 45/00 (2013.01); A61K 31/13 (2013.01); G01N 2333/70571 (2013.01); G01N 2800/2871 (2013.01); G01N 2800/50 (2013.01)] 3 Claims
 
1. A method for diagnosing a traumatic brain injury, the method comprising:
(a) obtaining a peripheral blood sample from a human subject;
(b) concentrating circulating brain microvascular endothelial cells from the peripheral blood sample sequentially by using a plurality of lectin coated magnetic beads and by using a plurality of magnetic beads coated with major facilitator superfamily domain-containing protein antibody;
(c) staining the concentrated circulating brain microvascular endothelial cells with a conjugated antibody, the conjugated antibody being a Fluorescein isothiocyanate (FITC) conjugated CD146 antibody;
(d) counting the number of the stained brain microvascular endothelial cells; and
(e) determining that the human subject has traumatic brain injury when a density of the circulating brain microvascular endothelial cells is greater than 90 count per milliliter.