US 11,054,413 B2
Materials and methods for assaying living cells
Stuart Martin, Severna Park, MD (US); Christopher M. Jewell, Silver Spring, MD (US); James I. Andorko, Delran, NJ (US); Elisabeth Lily Sooklal, Reisterstown, MD (US); Rebecca Whipple Bettes, Elkridge, MD (US); and Kristi Chakrabarti, Silver Spring, MD (US)
Assigned to UNIVERSITY OF MARYLAND, COLLEGE PARK, College Park, MD (US); and UNIVERSITY OF MARYLAND, BALTIMORE, Baltimore, MD (US)
Filed by Stuart Martin, Severna Park, MD (US); Christopher M. Jewell, Silver Spring, MD (US); James I. Andorko, Delran, NJ (US); Elisabeth Lily Sooklal, Reisterstown, MD (US); Rebecca Whipple Bettes, Elkridge, MD (US); and Kristi Chakrabarti, Silver Spring, MD (US)
Filed on Oct. 7, 2015, as Appl. No. 14/877,864.
Application 14/877,864 is a continuation in part of application No. PCT/US2015/024207, filed on Apr. 3, 2015.
Claims priority of provisional application 62/068,034, filed on Oct. 24, 2014.
Claims priority of provisional application 61/974,753, filed on Apr. 3, 2014.
Prior Publication US 2016/0097769 A1, Apr. 7, 2016
Int. Cl. G01N 33/50 (2006.01); G01N 21/95 (2006.01); G01N 33/574 (2006.01); G02B 21/00 (2006.01)
CPC G01N 33/5026 (2013.01) [G01N 21/95 (2013.01); G01N 33/50 (2013.01); G01N 33/574 (2013.01); G02B 21/0028 (2013.01); G01N 2800/7028 (2013.01)] 31 Claims
 
1. A device for assaying living cells comprising a substrate, wherein the substrate comprises a tethering molecule, wherein the tethering molecule adheres to the substrate and is capable of directly interacting with a cell membrane of a cell, wherein the cell maintains a free-floating, non-adherent character when bound to the tethering molecule, wherein the device exhibits an optical clarity of at least about 90%, wherein the substrate is coated with one or more layers of one or more materials that substantially inhibit the cells from adhering to the substrate, wherein a surface of the substrate is capable of being fixed to the cell by a chemical fixative.