US 11,054,367 B2
Spectroscopy imaging and analysis of live cells
Hoi-Ying N. Holman, Oakland, CA (US); Kevin Loutherback, Berkeley, CA (US); and Liang Chen, Walnut Creek, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Appl. No. 15/766,594
Filed by The Regents of the University of California, Oakland, CA (US)
PCT Filed Oct. 11, 2016, PCT No. PCT/US2016/056454
§ 371(c)(1), (2) Date Apr. 6, 2018,
PCT Pub. No. WO2017/066198, PCT Pub. Date Apr. 20, 2017.
Claims priority of provisional application 62/240,459, filed on Oct. 12, 2015.
Prior Publication US 2018/0299374 A1, Oct. 18, 2018
Int. Cl. G01N 21/3563 (2014.01); G01N 21/35 (2014.01); G01N 21/03 (2006.01); G01N 27/02 (2006.01); B01L 3/00 (2006.01)
CPC G01N 21/3563 (2013.01) [B01L 3/5027 (2013.01); G01N 21/35 (2013.01); G01N 27/026 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0877 (2013.01); B01L 2300/161 (2013.01); G01N 21/031 (2013.01); G01N 2021/3595 (2013.01); G01N 2201/129 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for infrared chemical mapping, comprising:
receiving Fourier Transform Infrared Spectroscopy (FTIR) data comprising FTIR spectra of samples collected over a time duration;
correcting scattering effects in the FTIR data; and
evaluating the FTIR spectra by performing an univariate analysis of the FTIR spectra or performing a multivariate analysis, comprising an unsupervised classification or a supervised classification, of the FTIR spectra,
wherein performing the univariate analysis comprises:
displaying a map of intensity distributions of the FTIR spectra at a single wavelength;
performing peak integration of the FTIR spectra; or
determining peak ratios of the FTIR spectra.