US 11,053,544 B2
Corn event DAS-59122-7 and methods for detection thereof
James Wayne Bing, Ankeny, IA (US); Robert F. Cressman, Jr., Wilmington, DE (US); Manju Gupta, Carmel, IN (US); Salim M. Hakimi, Sacramento, CA (US); David Hondred, Altoona, IA (US); Todd L. Krone, Johnston, IA (US); Mary E. Hartnett Locke, Mickleton, NJ (US); Abigail K. Luckring, West Chester, PA (US); Sandra E. Meyer, Des Moines, IA (US); Daniel Moellenbeck, Granger, IA (US); Kenneth Edwin Narva, Zionsville, IN (US); Paul D. Olson, Kalaheo, HI (US); Craig D. Sanders, Bear, DE (US); Jimei Wang, Johnston, IA (US); Jian Zhang, Urbandale, IA (US); and Gan-Yuan Zhong, Urbandale, IA (US)
Assigned to E. I. DuPont de Nemours and Company, Wilmington, DE (US); Pioneer Hi-Bred International, Inc., Johnston, IA (US); and Dow Agrosciences LLC, Indianapolis, IN (US)
Filed by E. I. DuPont de Nemours and Company, Wilmington, DE (US); Pioneer Hi-Bred International, Inc., Johnston, IA (US); and Dow Agrosciences LLC, Indianapolis, IN (US)
Filed on Oct. 2, 2017, as Appl. No. 15/722,697.
Application 11/938,483 is a division of application No. 11/237,222, filed on Sep. 28, 2005, granted, now 7,323,556, issued on Jan. 29, 2008.
Application 15/722,697 is a continuation of application No. 14/577,063, filed on Dec. 19, 2014, granted, now 9,879,321.
Application 14/577,063 is a continuation of application No. 11/938,483, filed on Nov. 12, 2007, granted, now 8,952,223, issued on Feb. 10, 2015.
Claims priority of provisional application 60/614,225, filed on Sep. 29, 2004.
Prior Publication US 2018/0057878 A1, Mar. 1, 2018
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6876 (2018.01); C12Q 1/6895 (2018.01); C07H 21/04 (2006.01); C12N 15/82 (2006.01); C07K 14/415 (2006.01)
CPC C12Q 1/6876 (2013.01) [C07K 14/415 (2013.01); C12N 15/8277 (2013.01); C12N 15/8286 (2013.01); C12Q 1/6895 (2013.01); C12Q 2600/13 (2013.01); Y02A 40/146 (2018.01)] 2 Claims
 
1. A method of detecting the presence of the DAS-59122-7 event insertion in corn tissue comprising:
(a) contacting a sample of said corn tissue with a polynucleotide probe unique to the event that hybridizes under stringent hybridization conditions with one or more DNA sequence selected from the group consisting of SEQ ID NO: 32, 33, 34, and 35 and complements thereof;
(b) subjecting said sample and probe to stringent hybridization conditions; and
(c) analyzing for hybridization of the probe.