US 11,053,539 B2
Detection substrate and manufacturing method thereof, and nucleic acid detecting method
Fengchun Pang, Beijing (CN); Peizhi Cai, Beijing (CN); and Yue Geng, Beijing (CN)
Assigned to BOE Technology Group Co., Ltd., Beijing (CN); and Beijing BOE Optoelectronics Technology Co., Ltd., Beijing (CN)
Appl. No. 15/750,907
Filed by BOE Technology Group Co., Ltd., Beijing (CN); and Beijing BOE Optoelectronics Technology Co., Ltd., Beijing (CN)
PCT Filed Jul. 12, 2017, PCT No. PCT/CN2017/092594
§ 371(c)(1), (2) Date Feb. 7, 2018,
PCT Pub. No. WO2018/099088, PCT Pub. Date Jun. 7, 2018.
Claims priority of application No. 201611091734.0 (CN), filed on Dec. 1, 2016.
Prior Publication US 2020/0087721 A1, Mar. 19, 2020
Int. Cl. B01L 3/00 (2006.01); C12Q 1/6869 (2018.01); C12Q 1/6825 (2018.01)
CPC C12Q 1/6869 (2013.01) [C12Q 1/6825 (2013.01); C12Q 2525/191 (2013.01); C12Q 2531/113 (2013.01); C12Q 2535/122 (2013.01); C12Q 2563/107 (2013.01); C12Q 2565/607 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A detection substrate comprising at least one detection unit disposed on a base substrate, the at least one detection unit comprising:
a first electrode;
a first passivation layer, located on the first electrode;
a second electrode, located on the first passivation layer, the second electrode comprising a plurality of sub-electrodes arranged in an array, the second electrode and the first electrode being insulated from each other, and the first electrode and the second electrode being configured to be applied with voltages, respectively;
a second passivation layer, located on the second electrode, the second passivation layer covering and filling in between the sub-electrodes arranged in the array; and
a colloid layer, located on the second passivation layer and at least disposed in a region corresponding to a region enclosed by adjacent sub-electrodes of the plurality of sub-electrodes, the colloid layer comprising a linker configured to be paired with a target nucleic acid,
wherein the first electrode is configured to be applied with a voltage having a polarity opposite to a polarity of an electric charge of the target nucleic acid, and the second electrode is configured to be applied with a voltage having a polarity the same as the polarity of the electric charge of the target nucleic acid.
 
10. A nucleic acid detecting method using a detection substrate,
the detection substrate comprising at least one detection unit disposed on a base substrate, the at least one detection unit comprising:
a first electrode;
a first passivation layer, located on the first electrode;
a second electrode, located on the first passivation layer, the second electrode comprising a plurality of sub-electrodes arranged in an array, the second electrode and the first electrode being insulated from each other, and the first electrode and the second electrode being configured to be applied with voltages, respectively;
a second passivation layer, located on the second electrode, the second passivation layer covering and filling in between the sub-electrodes arranged in the array; and
a colloid layer, located on the second passivation layer and at least disposed in a region corresponding to a region enclosed by adjacent sub-electrodes, the colloid layer comprising a linker configured to be paired with a target nucleic acid,
wherein the first electrode is configured to be applied with a voltage having a polarity opposite to a polarity of an electric charge of the target nucleic acid, and the second electrode is configured to be applied with a voltage having a polarity the same as the polarity of the electric charge of the target nucleic acid,
the nucleic acid detecting method comprising:
taking the at least one detection unit as an active unit, applying voltages with different polarities to the first electrode and the second electrode of the active unit, respectively, the voltage applied to the first electrode of the active unit having a polarity opposite to the polarity of the electric charge of the target nucleic acid;
flowing a library through the detection substrate, the linker in the active unit being paired with a target nucleic acid of the library, and performing an amplification to form a target nucleic acid to be detected; and
detecting the target nucleic acid to be detected.