US 11,053,524 B2
Microorganisms having putrescine productivity and process for producing putrescine using the same
Kyoung Min Lee, Seoul (KR); Hee Kyoung Jung, Seoul (KR); Young Lyeol Yang, Gyeonggi-do (KR); Hye Won Um, Gyeonggi-do (KR); Chang Gyeom Kim, Seoul (KR); Hong Xian Li, Seoul (KR); Su Jin Park, Seoul (KR); Jong Hyun Yoon, Gyeonggi-do (KR); Baek Seok Lee, Seoul (KR); and Sun Young Lee, Daejeon (KR)
Assigned to CJ CHEILJEDANG CORPORATION, Seoul (KR)
Filed by CJ CHEILJEDANG CORPORATION, Seoul (KR)
Filed on Jan. 11, 2019, as Appl. No. 16/246,082.
Application 16/246,082 is a continuation of application No. 14/778,383, granted, now 10,221,433, previously published as PCT/KR2014/001509, filed on Feb. 25, 2014.
Claims priority of application No. 10-2013-0030020 (KR), filed on Mar. 20, 2013; and application No. 10-2014-0017243 (KR), filed on Feb. 14, 2014.
Prior Publication US 2019/0136274 A1, May 9, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 13/00 (2006.01); C12N 9/88 (2006.01); C12N 9/10 (2006.01); C12N 9/02 (2006.01); C12N 9/12 (2006.01); C12N 1/21 (2006.01); C12N 15/52 (2006.01)
CPC C12P 13/001 (2013.01) [C12N 9/0008 (2013.01); C12N 9/1018 (2013.01); C12N 9/1029 (2013.01); C12N 9/1096 (2013.01); C12N 9/1217 (2013.01); C12N 9/88 (2013.01); C12N 15/52 (2013.01); C12Y 102/01038 (2013.01); C12Y 201/03003 (2013.01); C12Y 203/01035 (2013.01); C12Y 206/01011 (2013.01); C12Y 207/02008 (2013.01); C12Y 401/01007 (2013.01)] 11 Claims
 
1. A method for producing putrescine, comprising:
(i) culturing a recombinant microorganism having putrescine productivity in a culture medium, and
(ii) recovering putrescine from the cultured recombinant microorganism or the culture medium,
wherein the recombinant microorganism is modified to have enhanced activity of a protein having the amino acid sequence of SEQ ID NO: 21 or 23 as compared to the activity in the same microorganism without the modification,
wherein the activity of the protein is enhanced by increasing copy number of a polynucleotide encoding the protein or replacing a promoter of an endogenous polynucleotide encoding the protein with a stronger promoter.