US 11,053,512 B2
Methods of engineered tissue succulence in plants
John C. Cushman, Reno, NV (US); and Sung Don Lim, Reno, NV (US)
Assigned to BOARD OF REGENTS OF THE NEVADA SYSTEM OF HIGHER EDUCATION, ON BEHALF OF THE UNIVERSITY OF NEVADA, RENO, Reno, NV (US)
Filed by BOARD OF REGENTS OF THE NEVADA SYSTEM OF HIGHER EDUCATION ON BEHALF OF THE UNIVERSITY OF NEVADA, Reno, NV (US)
Filed on May 11, 2018, as Appl. No. 15/977,930.
Application 15/977,930 is a continuation in part of application No. PCT/US2016/061677, filed on Nov. 11, 2016.
Claims priority of provisional application 62/255,158, filed on Nov. 13, 2015.
Prior Publication US 2018/0327772 A1, Nov. 15, 2018
Int. Cl. C12N 15/82 (2006.01); C07K 14/415 (2006.01)
CPC C12N 15/8273 (2013.01) [C07K 14/415 (2013.01); C12N 15/8201 (2013.01); C12N 15/8225 (2013.01); C12N 15/8226 (2013.01); C12N 15/8234 (2013.01); C12N 15/8279 (2013.01); C12N 15/8294 (2013.01)] 15 Claims
 
1. A method of enhancing plant succulence, comprising:
inserting into a vector construct a nucleic acid sequence with at least 95% sequence identity to any one of SEQ ID NOS: 3-9 encoding a basic helix-loop-helix transcription factor cell elongation bHLH protein (CEB1);
transforming a plant cell with the vector construct;
expressing the CEB1 which is encoded by the nucleic acid sequence with at least 95% sequence identity to any one of SEQ ID NOS: 3-9 in the plant cell; and
producing a plant from the transformed plant cell, wherein the plant has enhanced plant succulence as compared to a control plant lacking the vector construct, wherein the plant having enhanced plant succulence has one or more of reduced hypocotyl length as compared to the control plant, reduced plant leaf water loss as compared to the control plant, reduced leaf stomatal aperture as compared to the control plant and reduced leaf stomatal density as compared to the control plant.