US 11,053,509 B2
Plant-produced chimaeric Orbivirus VLPs
Albertha René Van Zyl, Wynberg (ZA); Ann Elizabeth Meyers, Plumstead (ZA); Daria Anna Rutkowska, Centurion (ZA); Edward Peter Rybicki, Cape Town (ZA); Hester Catharina Stark, Irene (ZA); Martha Magaretha O'Kennedy, Pretoria (ZA); and Nobalanda Betty Mokoena, Montana (ZA)
Assigned to CSIR, Pretoria (ZA); UNIVERSITY OF CAPE TOWN, Cape Town (ZA); and ONDERSTEPOORT BIOLOGICAL PRODUCTS SOC LTD, Pretoria (ZA)
Appl. No. 16/95,264
Filed by CSIR, Pretoria (ZA); UNIVERSITY OF CAPE TOWN, Cape Town (ZA); and ONDERSTEPOORT BIOLOGICAL PRODUCTS SOC LTD, Pretoria (ZA)
PCT Filed Apr. 19, 2017, PCT No. PCT/IB2017/052236
§ 371(c)(1), (2) Date Oct. 19, 2018,
PCT Pub. No. WO2017/182958, PCT Pub. Date Oct. 26, 2017.
Claims priority of application No. 2016/02705 (ZA), filed on Apr. 19, 2016.
Prior Publication US 2019/0136246 A1, May 9, 2019
Int. Cl. A61K 39/12 (2006.01); C12N 15/82 (2006.01); A61P 31/14 (2006.01); A61K 35/76 (2015.01); A61K 39/00 (2006.01)
CPC C12N 15/8205 (2013.01) [A61K 35/76 (2013.01); A61K 39/12 (2013.01); A61P 31/14 (2018.01); C12N 15/8258 (2013.01); A61K 2039/5258 (2013.01); C12N 2720/12134 (2013.01)] 9 Claims
 
1. A method of producing a chimaeric African Horse Sickness Virus (AHSV) VLP in a plant cell, the method comprising:
(i) providing codon-optimised nucleotide sequences encoding AHSV VP2, VP3, VP5 and VP7 structural proteins, wherein at least one of the VP2, VP3, VP5 and VP7 structural proteins is selected from a first AHSV serotype and at least one of the VP2, VP3, VP5 and VP7 structural proteins is selected from a second AHSV serotype of the same AHSV species;
(ii) cloning the codon-optimised nucleotide sequences into at least one expression vector adapted to express the structural proteins in a plant cell;
(iii) transforming or infiltrating the plant cell with the at least one expression vector of step (ii);
(iv) co-expressing the VP2, VP3, VP5 and VP7 structural proteins in the plant cell, such that the expressed structural proteins assemble to form the chimaeric AHSV VLP; and
(v) recovering the chimaeric AHSV VLP from the plant cell.