US 11,053,311 B2
Antibodies targeting CDH19 for melanoma
Shouhua Xiao, Foster City, CA (US); Zheng Pan, Fremont, CA (US); Dineli Wickramasinghe, San Francisco, CA (US); M. Shawn Jeffries, Indianapolis, IN (US); Chadwick Terence King, North Vancouver (CA); and Brian Mingtung Chan, Port Coquitlam (CA)
Assigned to AMGEN INC., Thousand Oaks, CA (US)
Filed by AMGEN INC., Thousand Oaks, CA (US)
Filed on Jan. 23, 2018, as Appl. No. 15/878,047.
Application 15/878,047 is a division of application No. 14/762,053, granted, now 9,920,121, previously published as PCT/EP2014/051551, filed on Jan. 27, 2014.
Claims priority of provisional application 61/785,119, filed on Mar. 14, 2013.
Claims priority of provisional application 61/756,977, filed on Jan. 25, 2013.
Prior Publication US 2018/0208654 A1, Jul. 26, 2018
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 39/00 (2006.01); C07K 16/28 (2006.01); A61K 47/68 (2017.01); C07K 16/30 (2006.01)
CPC C07K 16/28 (2013.01) [A61K 47/6803 (2017.08); A61K 47/6849 (2017.08); A61K 47/6865 (2017.08); C07K 16/3053 (2013.01); A61K 2039/505 (2013.01); C07K 2317/21 (2013.01); C07K 2317/33 (2013.01); C07K 2317/34 (2013.01); C07K 2317/55 (2013.01); C07K 2317/73 (2013.01); C07K 2317/77 (2013.01); C07K 2317/92 (2013.01)] 25 Claims
 
1. An isolated human antibody or antigen binding fragment thereof capable of binding to human cadherin 19 (CDH19) on the surface of a target cell, comprising a VH region comprising CDR-H1, CDR-H2 and CDR-H3 and a VL region comprising CDR-L1, CDR-L2 and CDR-L3 selected from the group consisting of:
(a) a VH region comprising CDR-H1 as set forth in SEQ ID NO: 124, CDR-H2 as set forth in SEQ ID NO: 125, CDR-H3 as set forth in SEQ ID NO: 126, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 292, CDR-L2 as set forth in SEQ ID NO: 293 and CDR-L3 as set forth in SEQ ID NO: 294,
(b) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 130, CDR-H2 as set forth in SEQ ID NO: 131, CDR-H3 as set forth in SEQ ID NO: 132, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 298, CDR-L2 as set forth in SEQ ID NO: 299 and CDR-L3 as set forth in SEQ ID NO: 300,
(c) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 136, CDR-H2 as set forth in SEQ ID NO: 137, CDR-H3 as set forth in SEQ ID NO: 138, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 304, CDR-L2 as set forth in SEQ ID NO: 305 and CDR-L3 as set forth in SEQ ID NO: 306,
(d) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 142, CDR-H2 as set forth in SEQ ID NO: 143, CDR-H3 as set forth in SEQ ID NO: 144, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 310, CDR-L2 as set forth in SEQ ID NO: 311 and CDR-L3 as set forth in SEQ ID NO: 312,
(e) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 148, CDR-H2 as set forth in SEQ ID NO: 149, CDR-H3 as set forth in SEQ ID NO: 150, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 316, CDR-L2 as set forth in SEQ ID NO: 317 and CDR-L3 as set forth in SEQ ID NO: 318,
(f) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 166, CDR-H2 as set forth in SEQ ID NO: 167, CDR-H3 as set forth in SEQ ID NO: 168, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 334, CDR-L2 as set forth in SEQ ID NO: 335 and CDR-L3 as set forth in SEQ ID NO: 336,
(g) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 124, CDR-H2 as set forth in SEQ ID NO: 125, CDR-H3 as set forth in SEQ ID NO: 915, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 292, CDR-L2 as set forth in SEQ ID NO: 293 and CDR-L3 as set forth in SEQ ID NO: 294,
(h) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 124, CDR-H2 as set forth in SEQ ID NO: 125, CDR-H3 as set forth in SEQ ID NO: 915, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 292, CDR-L2 as set forth in SEQ ID NO: 293 and CDR-L3 as set forth in SEQ ID NO: 928,
(i) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 124, CDR-H2 as set forth in SEQ ID NO: 125, CDR-H3 as set forth in SEQ ID NO: 915, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 292, CDR-L2 as set forth in SEQ ID NO: 293 and CDR-L3 as set forth in SEQ ID NO: 929,
(j) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 166, CDR-H2 as set forth in SEQ ID NO: 167, CDR-H3 as set forth in SEQ ID NO: 168, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 334, CDR-L2 as set forth in SEQ ID NO: 335 and CDR-L3 as set forth in SEQ ID NO: 942,
(k) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 166, CDR-H2 as set forth in SEQ ID NO: 167, CDR-H3 as set forth in SEQ ID NO: 168, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 334, CDR-L2 as set forth in SEQ ID NO: 335 and CDR-L3 as set forth in SEQ ID NO: 943,
(l) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 148, CDR-H2 as set forth in SEQ ID NO: 149, CDR-H3 as set forth in SEQ ID NO: 150, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 316, CDR-L2 as set forth in SEQ ID NO: 317 and CDR-L3 as set forth in SEQ ID NO: 937,
(m) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 148, CDR-H2 as set forth in SEQ ID NO: 149, CDR-H3 as set forth in SEQ ID NO: 150, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 316, CDR-L2 as set forth in SEQ ID NO: 317 and CDR-L3 as set forth in SEQ ID NO: 938,
(n) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 148, CDR-H2 as set forth in SEQ ID NO: 149, CDR-H3 as set forth in SEQ ID NO: 919, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 316, CDR-L2 as set forth in SEQ ID NO: 317 and CDR-L3 as set forth in SEQ ID NO: 938,
(o) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 142, CDR-H2 as set forth in SEQ ID NO: 143, CDR-H3 as set forth in SEQ ID NO: 144, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 310, CDR-L2 as set forth in SEQ ID NO: 311 and CDR-L3 as set forth in SEQ ID NO: 935,
(p) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 142, CDR-H2 as set forth in SEQ ID NO: 143, CDR-H3 as set forth in SEQ ID NO: 918, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 310, CDR-L2 as set forth in SEQ ID NO: 311 and CDR-L3 as set forth in SEQ ID NO: 935,
(q) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 142, CDR-H2 as set forth in SEQ ID NO: 143, CDR-H3 as set forth in SEQ ID NO: 918, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 310, CDR-L2 as set forth in SEQ ID NO: 311 and CDR-L3 as set forth in SEQ ID NO: 936,
(r) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 136, CDR-H2 as set forth in SEQ ID NO: 137, CDR-H3 as set forth in SEQ ID NO: 138, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 304, CDR-L2 as set forth in SEQ ID NO: 305 and CDR-L3 as set forth in SEQ ID NO: 933,
(s) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 136, CDR-H2 as set forth in SEQ ID NO: 137, CDR-H3 as set forth in SEQ ID NO: 917, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 304, CDR-L2 as set forth in SEQ ID NO: 305 and CDR-L3 as set forth in SEQ ID NO: 934,
(t) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 130, CDR-H2 as set forth in SEQ ID NO: 131, CDR-H3 as set forth in SEQ ID NO: 132, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 298, CDR-L2 as set forth in SEQ ID NO: 299 and CDR-L3 as set forth in SEQ ID NO: 930,
(u) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 130, CDR-H2 as set forth in SEQ ID NO: 131, CDR-H3 as set forth in SEQ ID NO: 916, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 298, CDR-L2 as set forth in SEQ ID NO: 299 and CDR-L3 as set forth in SEQ ID NO: 931, and
(v) a VH region comprising CDR-H1 as set forth in CDR-H1 as set forth in SEQ ID NO: 130, CDR-H2 as set forth in SEQ ID NO: 131, CDR-H3 as set forth in SEQ ID NO: 916, and a VL region comprising CDR-L1 as set forth in SEQ ID NO: 298, CDR-L2 as set forth in SEQ ID NO: 299 and CDR-L3 as set forth in SEQ ID NO: 932.