US 11,053,302 B2
Stabilized and autonomous antibody VH domain
Tobias Carl Cornvik, Singapore (SG); Ignacio Jose Asial, Singapore (SG); and Par Lennart Nordlund, Singapore (SG)
Assigned to Nanyang Technological University, Singapore (SG)
Appl. No. 15/524,731
Filed by Nanyang Technological University, Singapore (SG)
PCT Filed Nov. 4, 2015, PCT No. PCT/SG2015/050432
§ 371(c)(1), (2) Date May 5, 2017,
PCT Pub. No. WO2016/072938, PCT Pub. Date May 12, 2016.
Claims priority of application No. 10201407244P (SG), filed on Nov. 5, 2014.
Prior Publication US 2017/0320934 A1, Nov. 9, 2017
Int. Cl. C07K 16/00 (2006.01); C07K 16/32 (2006.01); A61K 47/68 (2017.01)
CPC C07K 16/00 (2013.01) [C07K 16/005 (2013.01); C07K 16/32 (2013.01); C07K 2317/40 (2013.01); C07K 2317/569 (2013.01); C07K 2317/80 (2013.01); C07K 2317/94 (2013.01)] 9 Claims
 
1. A synthetic single domain antibody, wherein the synthetic antibody is an immunoglobulin VH domain comprising the framework sequences selected from the group consisting of
(a) EVQLVESGGGLVQPGGSLRLSCAASGF (SEQ ID NO:23), WVRQAPGKGLEWVA (SEQ ID NO:24), ADSVKGRFTISADTSKNT-Xaa-YLQMNSLRAEDTAVYYC-Xaa (SEQ ID NO:25), and -Y-Xaa-GQGTLVTVSS (SEQ ID NO:26), wherein the synthetic antibody comprises at least one modification selected from the group consisting of A78V, S93V, S93G and W103R with the position numbering being according to the Rabat numbering scheme; and
(b) EVQLVESGGGLVQPGGSLRLS-Xaa-A-Xaa-SGF- (SEQ ID NO:27), WVRQAPGKGLEWVA (SEQ ID NO:24), ADSVKGRFTISADTSKNT-Xaa-YLQMNSLRAEDTAVYY-Xaa-Xaa (SEQ ID NO:28), and Y-Xaa-GQGTLVTVSS (SEQ ID NO:26), wherein the synthetic antibody comprises at least one modification selected from the group consisting of A78V, S93V, S93G and W103R and at least one modification selected from the group consisting of C22S, A24I, A24L and C92T, wherein at least one of C22S and C92T is present with the position numbering being according to the Rabat numbering scheme.