US 11,053,292 B2
GM-CSF and IL-4 conjugates, compositions, and methods related thereto
Jacques Galipeau, Atlanta, GA (US); and Jiusheng Deng, Snellville, GA (US)
Assigned to Emory University, Atlanta, GA (US); and Children's Healthcare of Atlanta, Inc., Atlanta, GA (US)
Filed by Emory University, Atlanta, GA (US); and Children's Healthcare of Atlanta, Inc., Atlanta, GA (US)
Filed on Oct. 2, 2018, as Appl. No. 16/149,372.
Application 15/491,777 is a division of application No. 14/436,491, granted, now 9,657,076, issued on May 23, 2017, previously published as PCT/US2013/066261, filed on Oct. 23, 2013.
Application 16/149,372 is a continuation of application No. 15/491,777, filed on Apr. 19, 2017, granted, now 10,093,709.
Claims priority of provisional application 61/717,129, filed on Oct. 23, 2012.
Prior Publication US 2019/0016772 A1, Jan. 17, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/54 (2006.01); A61K 38/00 (2006.01); C12N 5/078 (2010.01); C12N 15/24 (2006.01); C12N 15/27 (2006.01); C12N 15/63 (2006.01); A61K 38/19 (2006.01); C07K 14/535 (2006.01); A61K 39/00 (2006.01); A61K 39/39 (2006.01); C12N 15/09 (2006.01); C12N 5/07 (2010.01); C12N 15/10 (2006.01); A61K 47/42 (2017.01); A61K 35/14 (2015.01); A61K 38/20 (2006.01); A61K 39/395 (2006.01)
CPC C07K 14/5406 (2013.01) [A61K 35/14 (2013.01); A61K 38/193 (2013.01); A61K 38/2026 (2013.01); A61K 39/0011 (2013.01); A61K 39/39 (2013.01); A61K 47/42 (2013.01); C07K 14/535 (2013.01); C12N 5/06 (2013.01); C12N 15/09 (2013.01); C12N 15/1024 (2013.01); C12N 15/1027 (2013.01); C12N 15/1062 (2013.01); A61K 38/00 (2013.01); A61K 2039/5152 (2013.01); A61K 2039/5156 (2013.01); A61K 2039/55522 (2013.01); A61K 2039/55527 (2013.01); C07K 2319/00 (2013.01)] 18 Claims
 
1. A conjugate comprising a granulocyte macrophage colony stimulating factor (GM-CSF) polypeptide and an interleukin 4 (IL-4) polypeptide, wherein said GM-CSF polypeptide and said IL-4 polypeptide are connected by a peptide linker consisting of amino acids selected from glycine, serine, threonine, asparagine, alanine and proline, wherein the conjugate is at least 70% identical to SEQ ID NO: 8, wherein said conjugate induces proliferation of B-cells.