US 11,053,285 B2
Nucleic acids encoding human immunodeficiency virus type 1 (HIV-1) N-terminal deleted gp120 immunogens and methods of use
Jerome Kim, Fort Detrick, MD (US); Stephen Harrison, Boston, MA (US); Barton F. Haynes, Durham, NC (US); Georgia D. Tomaras, Durham, NC (US); and Nelson Michael, Fort Detrick, MD (US)
Assigned to Duke University, Durham, NC (US); THE GOVERNMENT OF THE UNITED STATES, AS REPRESENTED BY THE SECRETARY OF THE ARMY, Fort Detrick, MD (US); and Children's Medical Center Corporation, Boston, MA (US)
Filed by Duke University, Durham, NC (US); The Government of the United States, as represented by the Secretary of the Army, Fort Detrick, MD (US); and Children's Medical Center Corporation, Boston, MA (US)
Filed on Jun. 27, 2018, as Appl. No. 16/20,543.
Application 16/020,543 is a division of application No. 14/130,338, granted, now 10,040,826, previously published as PCT/US2012/045530, filed on Jul. 5, 2012.
Claims priority of provisional application 61/529,137, filed on Aug. 30, 2011.
Claims priority of provisional application 61/457,906, filed on Jul. 5, 2011.
Prior Publication US 2018/0305411 A1, Oct. 25, 2018
Int. Cl. C07K 14/16 (2006.01); C07K 14/005 (2006.01); A61K 39/12 (2006.01); A61K 39/21 (2006.01); C07K 16/10 (2006.01); A61K 39/00 (2006.01); A61K 38/00 (2006.01)
CPC C07K 14/005 (2013.01) [A61K 39/12 (2013.01); A61K 39/21 (2013.01); C07K 14/162 (2013.01); C07K 16/1063 (2013.01); A61K 38/00 (2013.01); A61K 2039/6075 (2013.01); C07K 2317/21 (2013.01); C07K 2317/55 (2013.01); C07K 2317/92 (2013.01); C07K 2319/40 (2013.01); C12N 2710/16622 (2013.01); C12N 2740/16122 (2013.01); C12N 2740/16134 (2013.01)] 25 Claims
 
1. A nucleic acid encoding a recombinant protein comprising an HIV-1 envelope (Env) gp120 or gp140, wherein the original 4 to 25 consecutive amino acids of the N-terminus are deleted, wherein the deleted consecutive amino acids are located immediately after the envelope signal peptide and wherein an N-terminal Herpes Simplex gD tag is not substituted for amino acids of the N-terminus.